Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:336094
Zheng H, et al., CHIP inhibits odontoblast differentiation through promoting DLX3 polyubiquitylation and degradation. Development. 2023 May 15;150(10):dev200848
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7532785
|
|
Gene symbol:
|
Dlx3
|
|
Gene name:
|
distal-less homeobox 3
|
|
|
Results
|
Specimen
5C left:
postnatal day 1
(more  )
Note: An in situ proximity ligation assay (PLA) using Stub1 (CHIP) and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
Specimen
5C left:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
An in situ proximity ligation assay (PLA) using Stub1 (CHIP) and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: molar mesenchyme |
mesenchymal cell |
Present |
Regionally restricted |
5C left
|
Proximity ligation assay indicated that Stub1 interacted with Dlx3 in undifferentiated dental mesenchymal cells.
|
Specimen
5C right:
postnatal day 1
(more )
Note: An in situ proximity ligation assay (PLA) using Stub1 (CHIP) and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
Specimen
5C right:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
An in situ proximity ligation assay (PLA) using Stub1 (CHIP) and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: molar |
preodontoblast |
Present |
|
5C right
|
Proximity ligation assay indicated that Stub1 interacted with Dlx3 in preodontoblasts of P1 molars.
|
Specimen
5E left:
postnatal day 1
(more )
Note: An in situ proximity ligation assay (PLA) using ubiquitin and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
Specimen
5E left:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
An in situ proximity ligation assay (PLA) using ubiquitin and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: molar mesenchyme |
mesenchymal cell |
Present |
Regionally restricted |
5E left
|
In situ proximity ligation assay indicated that ubiquitylated DLX3 was present in the undifferentiated dental mesenchymal cells.
|
Specimen
5E right:
postnatal day 1
(more )
Note: An in situ proximity ligation assay (PLA) using ubiquitin and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
Specimen
5E right:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
An in situ proximity ligation assay (PLA) using ubiquitin and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: molar |
preodontoblast |
Present |
|
5E right
|
In situ proximity ligation assay indicated that ubiquitylated DLX3 was present in the preodontoblasts.
|
Specimen
7Fa:
postnatal day 14
(more )
Note: An in situ proximity ligation assay (PLA) using Stub1 (CHIP) and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
Specimen
7Fa:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal day 14
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
An in situ proximity ligation assay (PLA) using Stub1 (CHIP) and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: incisor mesenchyme |
mesenchymal cell |
Present |
Regionally restricted |
7Fa
|
Proximity ligation assay indicated that Stub1 interacted with Dlx3 in undifferentiated dental mesenchymal cells.
|
Specimen
7Fb:
postnatal day 14
(more )
Note: An in situ proximity ligation assay (PLA) using Stub1 (CHIP) and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
Specimen
7Fb:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal day 14
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
An in situ proximity ligation assay (PLA) using Stub1 (CHIP) and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: incisor |
odontoblast |
Weak |
Regionally restricted |
7Fb
|
Proximity ligation assay indicated that Stub1-Dlx3 complexes were decreased in secretory odontoblasts.
|
Specimen
7Fc:
postnatal day 14
(more )
Note: An in situ proximity ligation assay (PLA) using MDM2 and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
Specimen
7Fc:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal day 14
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
An in situ proximity ligation assay (PLA) using MDM2 and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: incisor mesenchyme |
mesenchymal cell |
Weak |
Regionally restricted |
7Fc
|
Proximity ligation assay indicated that the interaction between MDM2 and DLX3 was weak in undifferentiated dental mesenchymal cells.
|
Specimen
7Fd:
postnatal day 14
(more )
Note: An in situ proximity ligation assay (PLA) using MDM2 and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
Specimen
7Fd:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal day 14
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
An in situ proximity ligation assay (PLA) using MDM2 and DLX3 antibodies. Red fluorescent signals represent the presence of protein interaction.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: incisor |
odontoblast |
Present |
Regionally restricted |
7Fd
|
Proximity ligation assay indicated that the interaction between MDM2 and DLX3 was enhanced in the secretory odontoblasts.
|
|
Analysis Tools