Antibody:	anti-VGAT
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: spinal cord gray matter	Present		1C
TS28: spinal cord white matter	Absent		1C	No expression in axon bundles in anterior, lateral and posterior funiculi.

Structure	Level	Pattern	Image	Note
TS28: spinal cord ventral horn	Present		1D	Density of dots of expression in ventral horn was much higher than for GAD expression.

Structure	Level	Pattern	Image	Note
TS18: neural tube marginal layer	Present		7A	Expression first detected within marginal zone.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	7B	Expression localized within white matter.

Structure	Level	Pattern	Image	Note
TS21: spinal cord white matter	Present		7C

Structure	Level	Pattern	Image	Note
TS23: spinal cord white matter	Present		7D
TS23: spinal cord ventral horn	Present		7D	Expression observed after E15.

Structure	Level	Pattern	Image	Note
TS25: spinal cord white matter	Present		7E
TS25: spinal cord ventral horn	Strong		7E	Expression markedly increased on E17.
TS25: spinal cord dorsal horn	Weak		7E	Weak expression on E17

Structure	Level	Pattern	Image	Note
TS18: neural tube marginal layer	Present	Regionally restricted	7F	Dots of expression detected within ventral part of marginal zone.

Structure	Level	Pattern	Image	Note
TS20: spinal cord marginal layer	Present	Regionally restricted	7G	Dots of expression detected within ventral part of marginal zone.

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventral horn	Present	Regionally restricted	7H	Few dots of expression on axons within ventral horn near white matter.

Structure	Level	Pattern	Image	Note
TS23: spinal cord ventral horn	Present	Regionally restricted	7I	Many dots of expression diffusely detected on axons within gray matter.

Structure	Level	Pattern	Image	Note
TS25: spinal cord ventral horn	Present	Regionally restricted	7J	Numerous dots of expression, often surrounding large cell bodies of motor neurons.

Structure	Level	Pattern	Image	Note
TS21: spinal cord white matter	Present	Regionally restricted	7K	Numerous dots of expression within developing posterior funiculus.

Structure	Level	Pattern	Image	Note
TS23: spinal cord dorsal horn	Trace	Regionally restricted	7L	Few dots of expression first detected on E15.

Structure	Level	Pattern	Image	Note
TS25: spinal cord dorsal horn	Present	Regionally restricted	7M	Dots of expression diffusely increased in density, but still lower in number than in ventral horn.