Antibody:	CCN5 antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS14: embryo	Present	Widespread	S1A CCN5	Widely expressed in tissues of ectodermal, mesodermal and endodermal origin. Expression in all embryonic organs.
TS14: future brain	Present		S1A CCN5

Structure	Level	Pattern	Image	Note
TS16: embryo	Present	Widespread		Widely expressed in tissues of ectodermal, mesodermal and endodermal origin. Expression in all embryonic organs.

Structure	Level	Pattern	Image	Note
TS18: embryo	Present	Widespread	S1B CCN5	Widely expressed in tissues of ectodermal, mesodermal and endodermal origin. Expression in all embryonic organs.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present		1a CCN5	Differences in expression from Ccn2 beginning at E12.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present		1b CCN5	Expression in larger number of cell types than Ccn2.

Structure	Level	Pattern	Image	Note
TS24: embryo	Present		1c CCN5	Expression in larger number of cell types than Ccn2.

Structure	Level	Pattern	Image	Note
TS24: lung vascular element	Strong		2a CCN5	Abundantly expressed in endothelium and smooth muscle of veins and arteries.

Structure	Level	Pattern	Image	Note
TS20: heart apex	Strong		2b CCN5	Higher levels of expression in apex of heart when compared to myocardial expression levels.
TS20: cardiac muscle tissue	Present	Uniform	2b CCN5
TS20: heart septum	Weak	Regionally restricted	2b CCN5	Expression at lower levels in atrioventricular septum than in myocardium.

Structure	Level	Pattern	Image	Note
TS22: heart apex	Strong		2c CCN5	Higher levels of expression in apex of heart when compared to myocardial expression levels.
TS22: cardiac muscle tissue	Present		2c CCN5

Structure	Level	Pattern	Image	Note
TS22: heart apex	Strong		2d CCN5	Higher levels of expression in apex of heart when compared to myocardial expression levels.
TS22: cardiac muscle tissue	Present		2d CCN5
TS22: heart valve	Trace		2d CCN5	Very low expression in cardiac valves.
TS22: dorsal aorta	Weak		2d CCN5	Expression levels lower in aorta compared to pulmonary arterial trunk.
TS22: pulmonary artery	Present		2d CCN5

Structure	Level	Pattern	Image	Note
TS22: umbilical blood vessel	Present		2e CCN5

Structure	Level	Pattern	Image	Note
TS24: lung vascular element	Strong		S2A CCN5	Abundantly expressed in endothelium and smooth muscle of veins and arteries.

Structure	Level	Pattern	Image	Note
TS24: renal small blood vessel	Strong		S2B CCN5	Abundantly expressed in endothelium and smooth muscle of veins and arteries.

Structure	Level	Pattern	Image	Note
TS24: cardiac muscle tissue	Present	Uniform	S2C CCN5

Structure	Level	Pattern	Image	Note
TS22: bronchus epithelium	Present		3a CCN5

Structure	Level	Pattern	Image	Note
TS22: bronchiole epithelium	Present		3a CCN5 inset

Structure	Level	Pattern	Image	Note
TS24: bronchiole epithelium	Present	Regionally restricted	3b CCN5	Expression in terminal ends of all actively branching broncholes.
TS24: lung mesenchyme	Present	Regionally restricted	3b CCN5	Expression in mesenchyme surrounding branching bronchioles.

Structure	Level	Pattern	Image	Note
TS24: bronchus epithelium	Present	Regionally restricted	3c CCN5	Expression in epithelial and mesenchymal cell layers of larger bronchi that are no longer branching.
TS24: bronchus mesenchyme	Present	Regionally restricted	3c CCN5	Expression in epithelial and mesenchymal cell layers of larger bronchi that are no longer branching.

Structure	Level	Pattern	Image	Note
TS22: skeletal muscle	Strong	Regionally restricted	4a CCN5	Particularly strong expression at myotendinous junctions, beginning at E14.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: skeletal muscle		Strong	Regionally restricted	4b CCN5	Particularly strong expression at myotendinous junctions, beginning at E14.
TS24: epiphyseal plate hypertrophic zone	growth plate cartilage chondrocyte	Present		4b CCN5	Expression in chondrocytes during endochondral ossification. Expression in proliferating, hypertrophic and calcification zones.
TS24: epiphyseal plate ossification zone	growth plate cartilage chondrocyte	Present		4b CCN5	Expression in chondrocytes during endochondral ossification. Expression in proliferating, hypertrophic and calcification zones.
TS24: epiphyseal plate proliferative zone	growth plate cartilage chondrocyte	Present		4b CCN5	Expression in chondrocytes during endochondral ossification. Expression in proliferating, hypertrophic and calcification zones.
TS24: epiphyseal plate resting zone		Absent		4b CCN5	No expression in hyaline cartilage in resting zone.
TS24: artery endothelium		Strong		4b CCN5	Abundantly expressed in endothelium and smooth muscle of veins and arteries.
TS24: artery smooth muscle		Strong		4b CCN5	Abundantly expressed in endothelium and smooth muscle of veins and arteries.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: rib	chondrocyte	Present	Regionally restricted	4c CCN5	Expression in chondrocytes during endochondral ossification.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: liver	hepatocyte	Present		5a CCN5
TS24: liver	neutrophil	Present		5a CCN5
TS24: liver	hematopoietic stem cell	Absent		5a CCN5

Structure	Level	Pattern	Image	Note
TS20: small intestine epithelium	Weak		5b CCN5	Low levels of expression in intestinal epithelium and smooth muscle.
TS20: small intestine mesenchyme	Weak		5b CCN5	Low levels of expression in intestinal epithelium and smooth muscle.

Structure	Level	Pattern	Image	Note
TS22: intestine muscularis	Moderate		5c CCN5	Moderate levels of expression in intestinal epithelium and smooth muscle at E14.
TS22: small intestine epithelium	Moderate		5c CCN5	Moderate levels of expression in intestinal epithelium and smooth muscle at E14.

Structure	Level	Pattern	Image	Note
TS24: intestine muscularis	Present		5d CCN5	Intermediate levels of expression in intestinal epithelium and smooth muscle at E16.
TS24: small intestine epithelium	Present		5d CCN5	Intermediate levels of expression in intestinal epithelium and smooth muscle at E16.

Structure	Level	Pattern	Image	Note
TS20: metanephros	Present	Regionally restricted	6a CCN5	Expression in metanephric tubules in tubule terminals rather than uniformly throughout tubules.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: glomerular mesangium	glomerular mesangial cell	Present	Regionally restricted	6b CCN5
TS24: collecting duct		Present		6b CCN5	Expression in tubules, blood vessels and collecting ducts.
TS24: renal small blood vessel		Present		6b CCN5	Expression in tubules, blood vessels and collecting ducts.
TS24: renal tubule		Present		6b CCN5	Expression in tubules, blood vessels and collecting ducts.

Structure	Level	Pattern	Image	Note
TS24: glomerular capillary system of maturing glomerular tuft	Absent		6c CCN5	No expression in glomerular endothelial cells.

Structure	Level	Pattern	Image	Note
TS22: detrusor muscle of bladder	Present		6d CCN5	Expression in bladder smooth muscle, mesenchyme and urothelium.
TS22: urothelium of bladder	Present		6d CCN5	Expression in bladder smooth muscle, mesenchyme and urothelium.

Structure	Level	Pattern	Image	Note
TS24: detrusor muscle of bladder	Present		6e CCN5	Expression in urothelium and smooth muscle.
TS24: urothelium of bladder	Present		6e CCN5	Expression in urothelium and smooth muscle.

Structure	Level	Pattern	Image	Note
TS22: ovary	Present	Regionally restricted	6f CCN5	Expression in germinal epithelium of ovary.

Structure	Level	Pattern	Image	Note
TS24: ovary	Strong		6g CCN5	Higher levels of expression in ovary.
TS24: female reproductive system	Strong	Regionally restricted	6g CCN5	Higher levels of expression in epithelial cells and glands of reproductive tract.

Structure	Level	Pattern	Image	Note
TS24: thyroid gland	Moderate	Regionally restricted	7a CCN5	Moderate levels of expression in thyroid gland. Expression on colloidal side of thyroid follicles.

Structure	Level	Pattern	Image	Note
TS24: adrenal gland	Present	Regionally restricted	7b CCN5	Expression in connective tissue of adrenal capsule.

Structure	Level	Pattern	Image	Note
TS24: adrenal gland	Present	Regionally restricted	7b CCN5 inset	Expression in nuclei of some adrenal cells.

Structure	Level	Pattern	Image	Note
TS24: pancreatic duct	Weak			Low levels of expression in exocrine ducts.
TS24: spleen	Ambiguous			Absent or extremely low levels of expression in thymus and spleen.
TS24: thymus	Ambiguous			Absent or extremely low levels of expression in thymus and spleen.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Widespread	8a CCN5	Expression widely distributed in E12 brain.

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Regionally restricted	8b CCN5	Expression more restricted at E14.
TS22: choroid plexus	Present		8b CCN5

Structure	Level	Pattern	Image	Note
TS24: brain	Present	Regionally restricted	8c CCN5	Expression more restricted at E14.
TS24: choroid plexus	Present	Regionally restricted	8c CCN5

Structure	Cell Type	Level	Pattern	Image	Note
TS24: spinal cord	neuron	Strong	Regionally restricted	8d CCN5	Strong expression in nuclei of neurons.

Structure	Level	Pattern	Image	Note
TS24: vibrissa inner root sheath	Present		8e CCN5	Expression in both inner and outer root sheath.
TS24: vibrissa outer root sheath	Present		8e CCN5	Expression in both inner and outer root sheath.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Strong		8f CCN5

Structure	Level	Pattern	Image	Note
TS24: eyelid	Present		8g CCN5
TS24: cornea epithelium	Present		8g CCN5
TS24: cornea stroma	Present		8g CCN5