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Gene Expression Data
Assay Details
Assay
Reference: J:238642 Vrbacky M, et al., Knockout of Tmem70 alters biogenesis of ATP synthase and leads to embryonal lethality in mice. Hum Mol Genet. 2016 Nov 01;25(21):4674-4685
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7531852
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody coupled to rhodamine
Results
Specimen 4A +/+ left: embryonic day 9.5 (more )
Note: Double labeled: red - Acta2; green - Pecam1 (assay MGI:7531853).
Structure Cell Type Level Pattern Image Note
TS15: outflow tract fetal cardiomyocyte Present 4A +/+ left
TS15: primitive ventricle cardiac muscle fetal cardiomyocyte Present 4A +/+ left
Specimen 4A +/+ right: embryonic day 9.5 (more )
Note: Double labeled: red - Acta2; green - Pecam1 (assay MGI:7531853).
Structure Cell Type Level Pattern Image Note
TS15: outflow tract fetal cardiomyocyte Present 4A +/+ right
TS15: primitive ventricle cardiac muscle fetal cardiomyocyte Present 4A +/+ right
Specimen 4A +/- left: embryonic day 9.5; Tmem70tm1a(KOMP)Wtsi/Tmem70+ (more )
Note: Double labeled: red - Acta2; green - Pecam1 (assay MGI:7531853).
Structure Cell Type Level Pattern Image Note
TS15: atrium cardiac muscle fetal cardiomyocyte Present 4A +/- left Similar as in the wild-type.
TS15: primitive ventricle cardiac muscle fetal cardiomyocyte Present 4A +/- left Similar as in the wild-type.
Specimen 4A +/- right: embryonic day 9.5; Tmem70tm1a(KOMP)Wtsi/Tmem70+ (more )
Note: Double labeled: red - Acta2; green - Pecam1 (assay MGI:7531853).
Structure Cell Type Level Pattern Image Note
TS15: atrium cardiac muscle fetal cardiomyocyte Present 4A +/- right Similar as in the wild-type.
TS15: primitive ventricle cardiac muscle fetal cardiomyocyte Present 4A +/- right Similar as in the wild-type.
Specimen 4A -/- left: embryonic day 9.5; Tmem70tm1a(KOMP)Wtsi/Tmem70tm1a(KOMP)Wtsi (more )
Note: Double labeled: red - Acta2; green - Pecam1 (assay MGI:7531853).
Structure Cell Type Level Pattern Image Note
TS15: outflow tract fetal cardiomyocyte Present 4A -/- left Similar expression as in the wild-type, although with a smaller heart.
TS15: primitive ventricle cardiac muscle fetal cardiomyocyte Present 4A -/- left Similar expression as in the wild-type, although with a smaller heart.
Specimen 4A -/- right: embryonic day 9.5; Tmem70tm1a(KOMP)Wtsi/Tmem70tm1a(KOMP)Wtsi (more )
Note: Double labeled: red - Acta2; green - Pecam1 (assay MGI:7531853).
Structure Level Pattern Image Note
TS15: outflow tract Present 4A -/- right Similar expression as in the wild-type, with normal looping and were differentiated into proper compartments.
TS15: primitive ventricle cardiac muscle Present 4A -/- right Similar expression as in the wild-type, with normal looping and were differentiated into proper compartments.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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