Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:268103
Dykes IM, et al., A Requirement for Zic2 in the Regulation of Nodal Expression Underlies the Establishment of Left-Sided Identity. Sci Rep. 2018 Jul 11;8(1):10439
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7530986
|
|
Gene symbol:
|
Dand5
|
|
Gene name:
|
DAN domain family member 5, BMP antagonist
|
|
Probe:
|
cerl-2 probe1
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
4o:
embryonic day 8.0
(more  )
Note: 3-somite stage
Specimen
4o:
(close  )
|
Genetic Background:
|
involves: C3H/HeH * C57BL/6
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
3-somite stage
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Non-Uniform |
4o
|
Asymmetrically expressed at the node (L < R).
|
Specimen
4q:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 3-somite stage
Specimen
4q:
(close )
|
Genetic Background:
|
involves: C3H/HeH * C57BL/6
|
|
Mutant Allele(s):
|
Zic2iso/Zic2iso
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
3-somite stage
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Non-Uniform |
4q
|
Asymmetrically expressed at the node (L < R), similar as in the wild-type.
|
Specimen
4p:
embryonic day 8.0
(more )
Note: 2-somite stage
Specimen
4p:
(close )
|
Genetic Background:
|
involves: C3H/HeH * C57BL/6
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
2-somite stage
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Non-Uniform |
4p
|
Asymmetrically expressed at the node (L < R).
|
Specimen
4r:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 2-somite stage
Specimen
4r:
(close )
|
Genetic Background:
|
involves: C3H/HeH * C57BL/6
|
|
Mutant Allele(s):
|
Zic2iso/Zic2iso
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
2-somite stage
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Non-Uniform |
4r
|
Asymmetrically expressed at the node (L > R), which was different from the wild-type.
|
Specimen
not shown WT/Het:
embryonic day 8.0
(more )
Note: headfold and 1-4 somites, summarized in Fig 4s
Specimen
not shown WT/Het:
(close )
|
Genetic Background:
|
involves: C3H/HeH * C57BL/6
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
headfold and 1-4 somites, summarized in Fig 4s
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
|
|
Enriched on the right side of the node in 8 of 9 embryos: strong expression in 7 embryos, weak in 1. Symmetrically expressed (strong, L=R) in 1 out of 9 embryos.
|
Specimen
not shown iso/iso:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: headfold and 1-4 somites, summarized in Fig 4s
Specimen
not shown iso/iso:
(close )
|
Genetic Background:
|
involves: C3H/HeH * C57BL/6
|
|
Mutant Allele(s):
|
Zic2iso/Zic2iso
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
headfold and 1-4 somites, summarized in Fig 4s
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
|
|
Expression was maintained at wild-type levels (strong expression in 4 embryos, weak in 2), but the onset of its laterality appeared to be delayed and slightly perturbed: either L>R (in 2 embryos), L=R (in 2 embryos), or L<R (in 2 embryos).
|
|
Analysis Tools