Gene Expression Data
Assay Details
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Assay
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Reference:
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J:293110
Moreno-Mendoza N, et al., Disturbed expression of Sox9 in pre-sertoli cells underlies sex-reversal in mice b6.Ytir. Biol Reprod. 2004 Jan;70(1):114-22
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7530898
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Gene symbol:
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Sox9
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Gene name:
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SRY (sex determining region Y)-box 9
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Antibody:
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anti-Sox9
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
1A:
embryonic day 12.5
(more  )
Note: Cross section.
Specimen
1A:
(close  )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Cross section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: urogenital ridge |
Present |
Regionally restricted |
1A
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Expressing cells are located in the core of the genital ridge. They rarely form part of the coelomic epithelium.
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Specimen
1B:
embryonic day 12.5
(more )
Note: Longitudinal section.
Specimen
1B:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Longitudinal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: urogenital ridge |
Present |
Regionally restricted |
1B
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Expressing cells are located in the medial region. Expression is lacking in the cranial and caudal regions.
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Specimen
1C:
embryonic day 13.5
(more )
Note: Cross section.
Specimen
1C:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Cross section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: primary sex cord |
Present |
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1C
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Expressing cells are seen forming seminiferous cords.
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Specimen
1D:
embryonic day 13.5
(more )
Note: Cross section.
Specimen
1D:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Cross section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: gonad |
Present |
Single cells |
1D
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A few expressing cells are located on the dorsal side of the gonad, near the mesonephros.
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Specimen
1E:
embryonic day 13.5
(more )
Note: Tangential section.
Specimen
1E:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Tangential section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: gonad |
Present |
Single cells |
1E
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A few expressing cells are located on the dorsal side of the gonad, near the mesonephros.
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Specimen
1F:
embryonic day 13.5
(more )
Note: Longitudinal section.
Specimen
1F:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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Longitudinal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: primary sex cord |
Present |
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1F
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| TS21: gonad |
Present |
Single cells |
1F
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Scattered expressing cells are seen in the cranial region; none are seen in the caudal region.
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Specimen
4A:
embryonic day 18.5
(more )
Note: Cross section.
Specimen
4A:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Cross section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: gonad |
Present |
Regionally restricted |
4A
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This is an ovotestis. Scattered expressing cells are seen along the ventral, ovarian side of the gonad.
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| TS26: seminiferous cord |
Present |
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4A
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Specimen
4B:
embryonic day 18.5
(more )
Specimen
4B:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: ovary |
Absent |
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4B
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Specimen
4C:
embryonic day 18.5
(more )
Specimen
4C:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: gonad |
Present |
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4C
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This is an ovotestis. In this gonad the male tissue predominates over the ovarian tissue.
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| TS26: seminiferous cord |
Present |
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4C
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Specimen
4D:
embryonic day 18.5
(more )
Specimen
4D:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
Table 1 - E11.5:
embryonic day 11.5
(more )
Specimen
Table 1 - E11.5:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: gonad |
Present |
Single cells |
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For twelve (of 12) embryos, expression was seen in the gonad; in all cases it was bilateral.
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Specimen
Table 1 - E12.5:
embryonic day 12.5
(more )
Specimen
Table 1 - E12.5:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: gonad |
Present |
Single cells |
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For twelve (of 12) embryos, expression was seen in the gonad; in all cases it was bilateral.
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Specimen
Table 1 - E13.5:
embryonic day 13.5
(more )
Specimen
Table 1 - E13.5:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: gonad |
Present |
Single cells |
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For nine (of 12) embryos, expression was seen in the gonad; for 6 it was bilateral and for 3 it was unilateral.
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Specimen
Table 1 - E13.5:
embryonic day 13.5
(more )
Specimen
Table 1 - E13.5:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: gonad |
Absent |
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For three (of 12) embryos, no expression was seen in either gonad.
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Specimen
Table 1 - E15.5:
embryonic day 15.5
(more )
Specimen
Table 1 - E15.5:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: gonad |
Present |
Single cells |
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For seven (of 12) embryos, expression was seen in the gonad; for 5 it was bilateral and for 2 it was unilateral.
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Specimen
Table 1 - E15.5:
embryonic day 15.5
(more )
Specimen
Table 1 - E15.5:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: gonad |
Absent |
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For five (of 12) embryos, no expression was seen in either gonad.
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Specimen
Table 1 - E17.5:
embryonic day 17.5
(more )
Specimen
Table 1 - E17.5:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: gonad |
Present |
Single cells |
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For seven (of 12) embryos, expression was seen in the gonad; for 4 it was bilateral and for 3 it was unilateral.
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Specimen
Table 1 - E17.5:
embryonic day 17.5
(more )
Specimen
Table 1 - E17.5:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: gonad |
Absent |
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For five (of 12) embryos, no expression was seen in either gonad.
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Specimen
Table 1 - E18.5:
embryonic day 18.5
(more )
Specimen
Table 1 - E18.5:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
|
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: gonad |
Present |
Single cells |
|
For seven (of 12) embryos, expression was seen in the gonad; for 3 it was bilateral and for 4 it was unilateral.
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Specimen
Table 1 - E18.5:
embryonic day 18.5
(more )
Specimen
Table 1 - E18.5:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: gonad |
Absent |
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For five (of 12) embryos, no expression was seen in either gonad.
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