Antibody:	anti-DDX1 (2910)
Detection system:	colloidal gold

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present	Regionally restricted	1b DDX1 , 1b Magnified	Expression in aggregates localized to subplasmalemmal cytoplasm, many located next to mitochondria.

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present	Regionally restricted	S2a MII , S2a MII inset	Expression in aggregates. No expression in membrane bound vesicles.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Regionally restricted	S2a E0.5 , S2a E0.5 inset	Expression in membrane-bound vesicles first visualzed at 1-cell stage.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present	Regionally restricted	S2a E1.5 inset , S2a E1.5	Expression in membrane-bound vesicles.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present	Regionally restricted	S2a E2.5 inset , S2a E2.5	Expression in membrane-bound vesicles.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Regionally restricted	S2a E3.5 , S2a E3.5 inset	Expression in membrane-bound vesicles mainly surrounding nucleus.

Structure	Level	Pattern	Image	Note
TS6: blastocyst	Present	Regionally restricted	S2a Late Blastocyst inset , S2a Late Blastocyst	Expression in aggregates mainly surrounding nucleus. Membrane-bound vesicles no longer detected at E4.5.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present	Regionally restricted	S2b Inner Cell Mass , S2b Inner Cell Mass inset	Expression in membrane-bound vesicles in both inner cell mass and trophectoderm.

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Present	Regionally restricted	S2b Trophectoderm , S2b Trophectoderm inset	Expression in membrane-bound vesicles in both inner cell mass and trophectoderm.