Gene Expression Data
Assay Details
Assay
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Reference:
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J:340391
Pina JO, et al., Multimodal spatiotemporal transcriptomic resolution of embryonic palate osteogenesis. Nat Commun. 2023 Sep 14;14(1):5687
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7530041
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Gene symbol:
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Sparc
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Gene name:
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secreted acidic cysteine rich glycoprotein
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Probe:
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Mm-Sparc (466781)
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Probe preparation:
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Antisense DNA
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Visualized with:
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Fluorescence
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Results
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Specimen
4a Sparc E14.5:
embryonic day 14.5
(more )
Note: Multi-labeled: yellow - Sparc; green - Col1a1 (assay MGI:7530040); red - Runx2 (assay MGI:7530039); magenta - Sost (assay MGI:7530042).
Specimen
4a Sparc E14.5:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Multi-labeled: yellow - Sparc; green - Col1a1 (assay MGI:7530040); red - Runx2 (assay MGI:7530039); magenta - Sost (assay MGI:7530042).
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: palatal shelf mesenchyme |
osteoblast |
Present |
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4a E14.5
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Expression in mature functional osteoblasts. Expression throughout developing palatal mesenchyme.
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Specimen
4a Sparc E15.5:
embryonic day 15.5
(more )
Note: Multi-labeled: yellow - Sparc; green - Col1a1 (assay MGI:7530040); red - Runx2 (assay MGI:7530039); magenta - Sost (assay MGI:7530042).
Specimen
4a Sparc E15.5:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Multi-labeled: yellow - Sparc; green - Col1a1 (assay MGI:7530040); red - Runx2 (assay MGI:7530039); magenta - Sost (assay MGI:7530042).
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Specimen
4b Sparc E14.5:
embryonic day 14.5
(more )
Note: Double labeled: yellow - Sparc; red - Runx2 (assay MGI:7530039).
Specimen
4b Sparc E14.5:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: yellow - Sparc; red - Runx2 (assay MGI:7530039).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: palatal shelf mesenchyme |
osteoblast |
Present |
|
4b E14.5
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Expression in mature functional osteoblasts. Expression throughout developing palatal mesenchyme.
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Specimen
4b Sparc E15.5:
embryonic day 15.5
(more )
Note: Double labeled: yellow - Sparc; magenta - Sost (assay MGI:7530042).
Specimen
4b Sparc E15.5:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: yellow - Sparc; magenta - Sost (assay MGI:7530042).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: palatal shelf mesenchyme |
osteoblast |
Present |
Regionally restricted |
4b E15.5
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Expression in mature functional osteoblasts. Expression throughout developing palatal mesenchyme. Diffuse expression in central ossification centers.
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