Gene Expression Data
Assay Details
Assay
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Reference:
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J:85979
Muller-Decker K, et al., Expression of cyclooxygenase isozymes during morphogenesis and cycling of pelage hair follicles in mouse skin: precocious onset of the first catagen phase and alopecia upon cyclooxygenase-2 overexpression. J Invest Dermatol. 2003 Oct;121(4):661-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7526800
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Gene symbol:
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Ptgs2
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Gene name:
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prostaglandin-endoperoxide synthase 2
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Antibody:
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anti-COX-2 (sc-1745)
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
2A:
embryonic day 13.0
(more )
Specimen
2A:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS21: epidermis |
Present |
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2A
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Expression in epithelial precursor cells of embryonic epidermis prior to onset of epidermal stratification.
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Specimen
2B:
embryonic day 15.0
(more )
Specimen
2B:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: epidermis basal layer |
basal cell of epidermis |
Present |
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2B
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TS23: periderm |
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Present |
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2B
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Expression in outermost ectoderm periderm layer of stratified epidermis.
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TS23: epidermis |
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Present |
Regionally restricted |
2B
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No expression in differentiated layers in between.
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Specimen
2C:
embryonic day 18.0
(more )
Specimen
2C:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
2C inset:
embryonic day 18.0
(more )
Specimen
2C inset:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: hair germ |
Present |
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2C inset
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Expression in elongated hair germs and hair pegs at stages 2 and 3.
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TS26: hair peg |
Present |
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2C inset
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Expression in elongated hair germs and hair pegs at stages 2 and 3.
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Specimen
2D:
postnatal day 8
(more )
Specimen
2D:
(close )
Genetic Background:
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NMRI
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Age:
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postnatal day 8
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: hair outer root sheath |
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Present |
Regionally restricted |
2D
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Expression restricted to basal outer root sheath.
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TS28: hair follicle |
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Present |
Regionally restricted |
2D
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Expression in outer root sheath cells of distal and also proximal hair follicles.
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TS28: sebaceous gland |
basal cell of epidermis |
Present |
Regionally restricted |
2D
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Expression in basal cells of sebaceous gands.
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Specimen
2D inset:
postnatal day 8
(more )
Specimen
2D inset:
(close )
Genetic Background:
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NMRI
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Age:
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postnatal day 8
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: interfollicular epidermis |
basal cell of epidermis |
Present |
Regionally restricted |
2D inset
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Expression in basal interfollicular epidermis.
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Specimen
2E:
postnatal day 12
(more )
Specimen
2E:
(close )
Genetic Background:
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NMRI
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
2F:
postnatal day 17
(more )
Specimen
2F:
(close )
Genetic Background:
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NMRI
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Age:
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postnatal day 17
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: hair follicle |
Weak |
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2F
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Expression declined in catagen hair follicles at P17.
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Specimen
2G:
postnatal day 21
(more )
Specimen
2G:
(close )
Genetic Background:
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NMRI
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Age:
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postnatal day 21
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: hair follicle |
Trace |
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2G
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Expression barely detectable in telogen hair follicles at P21.
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Specimen
2H:
postnatal day 28
(more )
Specimen
2H:
(close )
Genetic Background:
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NMRI
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Age:
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postnatal day 28
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: hair outer root sheath |
Present |
Regionally restricted |
2H
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Expression in outer root sheath of late anagen hair follicles at P28.
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Specimen
2H inset:
postnatal day 25
(more )
Specimen
2H inset:
(close )
Genetic Background:
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NMRI
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Age:
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postnatal day 25
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: hair outer root sheath |
Present |
Regionally restricted |
2H inset
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Re-expression in outer root sheath of early anagen hair follicles at P25.
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Specimen
2I:
postnatal day 28
(more )
Specimen
2I:
(close )
Genetic Background:
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NMRI
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Age:
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postnatal day 28
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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