Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:315020
Yao G, et al., The cytoplasmic protein Pacsin 2 in kidney development and injury repair. Kidney Int. 2013 Mar;83(3):426-37
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:7526221
|
Gene symbol:
|
Pacsin2
|
Gene name:
|
protein kinase C and casein kinase substrate in neurons 2
|
|
Results
|
Specimen
1a newborn:
postnatal newborn
(more )
Note: Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
Specimen
1a newborn:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: renal proximal tubule |
epithelial cell of proximal tubule |
Strong |
|
1a upper right
,
1a upper left
|
Expressed in the apical membrane of Lotus tetragonolobus agglutinin positive proximal tubules.
|
Specimen
not shown 1 week:
postnatal week 1
(more )
Note: Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
Specimen
not shown 1 week:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal week 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: renal proximal tubule |
epithelial cell of proximal tubule |
Present |
|
|
Expressed in the apical membrane of Lotus tetragonolobus agglutinin positive proximal tubules.
|
Specimen
1a 3-weeks:
postnatal week 3
(more )
Note: Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
Specimen
1a 3-weeks:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal week 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: renal proximal tubule |
epithelial cell of proximal tubule |
Present |
|
1a lower left
,
1a lower right
|
Remarkably reduced expression and became mostly cytoplasmic, with only weak signals on the apical membrane in a small fraction (about 5%).
|
Specimen
1b newborn:
postnatal newborn
(more )
Note: Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
Specimen
1b newborn:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: renal distal tubule |
epithelial cell of distal tubule |
Strong |
|
1b upper left
,
1b upper right
|
Expressed in the apical membrane of Dolichos biflorus agglutinin positive distal tubules.
|
Specimen
not shown 1 week:
postnatal week 1
(more )
Note: Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
Specimen
not shown 1 week:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal week 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: renal distal tubule |
epithelial cell of distal tubule |
Present |
|
|
Expressed in the apical membrane of Dolichos biflorus agglutinin positive distal tubules.
|
Specimen
1b 3-weeks:
postnatal week 3
(more )
Note: Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
Specimen
1b 3-weeks:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal week 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: renal distal tubule |
epithelial cell of distal tubule |
Strong |
|
1b lower right
,
1b lower left
|
Even stronger expression in the apical membrane of Dolichos biflorus agglutinin positive distal tubules.
|
Specimen
2a1:
postnatal newborn
(more )
Note: Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
Specimen
2a1:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
|
Specimen
2a2/3:
postnatal newborn
(more )
Note: Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
Specimen
2a2/3:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
|
Specimen
2a4/5:
postnatal newborn
(more )
Note: Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
Specimen
2a4/5:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
|
Specimen
2b1/2:
postnatal newborn
(more )
Note: Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
Specimen
2b1/2:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
|
Specimen
2b3/4:
postnatal month 3
(more )
Note: Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
Specimen
2b3/4:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal month 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: glomerular tuft |
Present |
|
2b3
,
2b4
|
There was a remarkable upregulation.
|
Specimen
7a control:
postnatal month 3
(more )
Specimen
7a control:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal month 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: renal proximal tubule |
epithelial cell of proximal tubule |
Present |
|
7a upper left
|
Expressed at basal levels in the cytoplasm.
|
Specimen
7b control:
postnatal month 3
(more )
Specimen
7b control:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal month 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: renal distal tubule |
epithelial cell of distal tubule |
Present |
|
7b upper left
|
|
Specimen
S1 E15.5 upper:
embryonic day 15.5
(more )
Note: Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
Specimen
S1 E15.5 upper:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: cortical early proximal tubule |
epithelial cell of proximal tubule |
Present |
|
S1_E15.5 upper left
,
S1_E15.5 upper right
|
Expressed in the apical membrane of Lotus tetragonolobus agglutinin positive proximal tubules.
|
Specimen
S1 E15.5 lower:
embryonic day 15.5
(more )
Note: Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
Specimen
S1 E15.5 lower:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: cortical early distal tubule |
epithelial cell of distal tubule |
Present |
|
S1_E15.5 lower right
,
S1_E15.5 lower left
|
Expressed in the apical membrane of Dolichos biflorus agglutinin positive distal tubules.
|
Specimen
S1 E18.5 upper:
embryonic day 18.5
(more )
Note: Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
Specimen
S1 E18.5 upper:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: renal proximal tubule |
epithelial cell of proximal tubule |
Present |
|
S1_E18.5 upper left
,
S1_E18.5 upper right
|
Expressed in the apical membrane of Lotus tetragonolobus agglutinin positive proximal tubules.
|
Specimen
S1 E18.5 lower:
embryonic day 18.5
(more )
Note: Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
Specimen
S1 E18.5 lower:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: renal distal tubule |
epithelial cell of distal tubule |
Present |
|
S1_E18.5 lower left
,
S1_E18.5 lower right
|
Expressed in the apical membrane of Dolichos biflorus agglutinin positive distal tubules.
|
Specimen
S5a control:
postnatal month 3
(more )
Note: Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
Specimen
S5a control:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal month 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Lotus tetragonolobus agglutinin (LTA).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: renal proximal tubule |
epithelial cell of proximal tubule |
Weak |
|
S5a upper left
,
S5a upper right
|
|
Specimen
S5b control:
postnatal month 3
(more )
Note: Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
Specimen
S5b control:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal month 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pacsin2; green - Dolichos biflorus agglutinin (DBA).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: renal distal tubule |
epithelial cell of distal tubule |
Present |
|
S5b upper left
,
S5b upper right
|
|
|