Gene Expression Data
Assay Details
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Assay
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Reference:
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J:132979
Potok MA, et al., WNT signaling affects gene expression in the ventral diencephalon and pituitary gland growth. Dev Dyn. 2008 Apr;237(4):1006-20
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7526043
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Gene symbol:
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Tshb
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Gene name:
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thyroid stimulating hormone, beta subunit
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Antibody:
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anti-TSHbeta
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
2M:
embryonic day 16.5
(more  )
Specimen
2M:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2N:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4tm1Amc
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: adenohypophysis |
Weak |
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2N
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Slight reduction in expression compared to wild type.
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Specimen
4Be:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4+
Wnt5atm1Amc/Wnt5a+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4Bf:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4tm1Amc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4Bg:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4Bh:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4tm1Amc
Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: adenohypophysis |
thyrotroph |
Weak |
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4Bh
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Expression in thyrotropes slightly reduced compared to wild type.
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Specimen
5 TSHbeta +/+:
embryonic day 18.5
(more )
Specimen
5 TSHbeta +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5 TSHbeta -/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Wnt6tm1Akis/Wnt6tm1Akis
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools