Antibody:	anti-WNT5A
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	1A	Expression in ventral diencephalon, particularly in basal cells lining lumen, with no expression in cells on apical aspect.
TS17: Rathke's pouch	Present	Regionally restricted	1A	Expression in Rathke's pouch as early as E10.5 in cells lining presumptive lumen.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	1B	Expression in ventral diencephalon, throughout rostral and caudal domains.
TS19: Rathke's pouch	Present		1B

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	1C	Expression in ventral diencephalon throughtout rostral and caudal domains.
TS20: remnant of Rathke's pouch	Absent		1C

Structure	Level	Pattern	Image	Note
TS22: neurohypophysis pars nervosa	Present		1D	Expression in developing posterior lobe.
TS22: neurohypophysis infundibulum	Present		1D	Expression in infundibulum.
TS22: adenohypophysis	Present	Regionally restricted	1D	Expression in ventral region of anterior lobe, extending into rostral tip.

Structure	Level	Pattern	Image	Note
TS24: pituitary gland	Weak		1E	Expression waning by E16.5.

Structure	Level	Pattern	Image	Note
TS28: adenohypophysis pars anterior	Present		1F	Expression evident again in adult pituitary in anterior and intermediate lobes.
TS28: adenohypophysis pars intermedia	Present		1F	Expression evident again in adult pituitary in anterior and intermediate lobes.
TS28: neurohypophysis	Absent		1F	No expression in mature posterior lobe.