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Gene Expression Data
Assay Details
Assay
Reference: J:183829 Correa SM, et al., Sex reversal in C57BL/6J XY mice caused by increased expression of ovarian genes and insufficient activation of the testis determining pathway. PLoS Genet. 2012 Apr;8(4):e1002569
Assay type: RT-PCR
MGI Accession ID: MGI:7525410
Gene symbol: Nr5a1
Gene name: nuclear receptor subfamily 5, group A, member 1
Probe: Mm00446826_m1
Probe preparation: labelled with FAM
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh.
Results
Image: 5_Sf1 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
E10.5 D2 E10.5 TS17: gonad primordium
TS17: mesonephros 		Present Not Specified; total RNA DBA/2J Male Gonad-mesonephros complexes were used.
E10.5 B6 E10.5 TS17: gonad primordium
TS17: mesonephros 		Present (a) Not Specified; total RNA C57BL/6J Male Gonad-mesonephros complexes were used.
E11.0 D2 E11.0 TS18: gonad primordium
TS18: mesonephros 		Present Not Specified; total RNA DBA/2J Male Gonad-mesonephros complexes were used.
E11.0 B6 E11.0 TS18: gonad primordium
TS18: mesonephros 		Present (b) Not Specified; total RNA C57BL/6J Male Gonad-mesonephros complexes were used.
E11.5 D2 E11.5 TS19: gonad
TS19: mesonephros 		Present Not Specified; total RNA DBA/2J Male Gonad-mesonephros complexes were used.
E11.5 B6 E11.5 TS19: gonad
TS19: mesonephros 		Present (b) Not Specified; total RNA C57BL/6J Male Gonad-mesonephros complexes were used.
E12.0 D2 E12.0 TS20: mesonephros
TS20: testis 		Present Not Specified; total RNA DBA/2J Male Gonad-mesonephros complexes were used.
E12.0 B6 E12.0 TS20: mesonephros
TS20: testis 		Present (b) Not Specified; total RNA C57BL/6J Male Gonad-mesonephros complexes were used.
E12.5 D2 E12.5 TS20: mesonephros
TS20: testis 		Present Not Specified; total RNA DBA/2J Male Gonad-mesonephros complexes were used.
E12.5 B6 E12.5 TS20: mesonephros
TS20: testis 		Present (a) Not Specified; total RNA C57BL/6J Male Gonad-mesonephros complexes were used.
E13.5 D2 E13.5 TS21: mesonephros
TS21: testis 		Present Not Specified; total RNA DBA/2J Male Gonad-mesonephros complexes were used.
E13.5 B6 E13.5 TS21: mesonephros
TS21: testis 		Present (a) Not Specified; total RNA C57BL/6J Male Gonad-mesonephros complexes were used.
Notes:
(a) Similar as in D2.
(b) Expression was lower in B6 but not statistically significant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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