Probe:	Lhx1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS27: ventral lateral geniculate nucleus	Present		2I
TS27: zona incerta	Present	Regionally restricted	2I	Expression in rostral subdivision of zona incerta.

Structure	Level	Pattern	Image	Note
TS27: ventral lateral geniculate nucleus	Present	Non-Uniform	2J	Expression in ventral lateral geniculate nucleus is not uniform.
TS27: zona incerta	Present	Regionally restricted	2J	Expression in rostral subdivision of zona incerta.

Structure	Level	Pattern	Image	Note
TS27: ventral lateral geniculate nucleus	Present	Regionally restricted	2K	Relatively low expression in middle and dorsal parts.
TS27: zona incerta	Present	Regionally restricted	2K	Expression in rostral subdivision of zona incerta.

Structure	Level	Pattern	Image	Note
TS27: ventral lateral geniculate nucleus	Present	Regionally restricted	2L	Relatively low expression in middle and dorsal parts.
TS27: zona incerta	Present	Regionally restricted	2L	Expression in dorsal and ventral subdivisions of zona incerta.

Structure	Level	Pattern	Image	Note
TS24: ventral lateral geniculate nucleus	Strong	Regionally restricted	5E	Expression in ventral lateral geniculate nucleus is high in ventromedial and ventrolateral parts.
TS24: zona incerta	Present	Regionally restricted	5E	Expression in rostral, dorsal and ventral subdivisions of zona incerta
TS24: zona limitans intrathalamica	Present		5E

Structure	Level	Pattern	Image	Note
TS24: ventral lateral geniculate nucleus	Strong	Regionally restricted	5F	Expression in ventral lateral geniculate nucleus is high in ventromedial and ventrolateral parts.
TS24: zona limitans intrathalamica	Present		5F

Structure	Level	Pattern	Image	Note
TS24: ventral lateral geniculate nucleus	Strong	Regionally restricted	5G	Expression in ventral lateral geniculate nucleus is high in ventromedial and ventrolateral parts. Expression in ventral lateral geniculate nucleus is high ventrally.

Structure	Level	Pattern	Image	Note
TS24: zona incerta	Present	Regionally restricted	5H	Expression in rostral, dorsal and ventral subdivisions of zona incerta.
TS24: zona limitans intrathalamica	Present	Regionally restricted	5H	Expressed in two bands of zona limitans intrathalamica.

Structure	Level	Pattern	Image	Note
TS22: ventral lateral geniculate nucleus	Present		6A

Structure	Level	Pattern	Image	Note
TS22: ventral lateral geniculate nucleus	Strong	Regionally restricted	6B	Expression in ventral lateral geniculate nucleus is high ventrally.
TS22: zona limitans intrathalamica	Present	Regionally restricted	6B	Expressed in two bands of zona limitans intrathalamica.

Structure	Level	Pattern	Image	Note
TS22: zona limitans intrathalamica	Present		6C

Structure	Level	Pattern	Image	Note
TS22: zona limitans intrathalamica	Present		6D

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	7A' , 7A	Domain of expression surrounding thalamocortical axon bundle.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	7B , 7B'	At more caudal level, thalamocortical axon bundle does not express Lhx1. Domain expressing Lhx1, Isl1 and Pax6 but not Lhx5. Expression in putative ventral lateral geniculate nucleus.
TS20: zona limitans intrathalamica	Present		7B , 7B'

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	7C	At most caudal level, domain of expression in ventral lateral geniculate nucleus.
TS20: zona limitans intrathalamica	Present		7C

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	8F	Expression in ventral thalamus.
TS17: diencephalon mantle layer	Present	Regionally restricted	8F