Probe:	Lhx2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS27: anterior medial thalamic nucleus	Present		1K
TS27: central lateral thalamic nucleus	Present		1K

Structure	Level	Pattern	Image	Note
TS27: central lateral thalamic nucleus	Present		1L
TS27: central medial thalamic nucleus	Present		1L
TS27: ventral lateral thalamic nucleus	Present		1L

Structure	Level	Pattern	Image	Note
TS27: central lateral thalamic nucleus	Present		1M
TS27: paraventricular thalamic nucleus	Present		1M

Structure	Level	Pattern	Image	Note
TS27: zona incerta	Absent		1N

Structure	Level	Pattern	Image	Note
TS27: peripeduncular nucleus	Present		1O
TS27: medial geniculate nucleus	Present	Regionally restricted	1O	Expression in medial geniculate nucleus, particularly in the lateral part of ventral subdivision.

Structure	Level	Pattern	Image	Note
TS27: dorsal lateral geniculate nucleus	Absent			Ventroposterior nucleus and dorsal lateral geniculate nucleus do not express at detectable levels.
TS27: ventral posterior thalamic group	Absent			Ventroposterior nucleus and dorsal lateral geniculate nucleus do not express at detectable levels.

Structure	Level	Pattern	Image	Note
TS24: dorsal thalamus	Weak	Regionally restricted	3A	At rostral levels, only weakly expressed.

Structure	Level	Pattern	Image	Note
TS24: dorsal lateral geniculate nucleus	Absent		3B	At middle level, no expression in square-shaped region , compatible with dorsal lateral geniculate nucleus.
TS24: lateral posterior thalamic nucleus	Weak		3B	Putative lateral posterior nucleus expresses only very low levels of Lhx2.
TS24: ventral posterior thalamic group	Absent		3B	Putative ventroposterior nucleus is negative for Lhx2.
TS24: ventral medial thalamic nucleus	Absent		3B	Putative ventromedial nucleus is negative for Lhx2.

Structure	Level	Pattern	Image	Note
TS24: medial geniculate nucleus	Present	Regionally restricted	3C	At caudal level, ventral subdivision of medial geniculate nucleus is strongly positive for Lhx2 Dorsal subdivision of medial geniculate nucleus expresses Lhx2.
TS24: peripeduncular nucleus	Present		3C

Structure	Level	Pattern	Image	Note
TS22: lateral dorsal thalamic nucleus	Present		4A	At rostral level, Lhx2 not expressed in lateral-ventral part of dorsal thalamus, corresponding to laterodorsal nucleus.
TS22: dorsal thalamus	Strong	Regionally restricted	4A	Band of strong Lhx2 expression located more medially.

Structure	Level	Pattern	Image	Note
TS22: thalamus ventricular layer	Absent		4B	No expression in putative ventricular zone.
TS22: dorsal lateral geniculate nucleus	Absent		4B	More caudally, no expression in region expressing Lhx9 and Ngn2, likely to be dorsal lateral geniculate nucleus.
TS22: lateral posterior thalamic nucleus	Weak		4B	Low levels of Lhx2 expression in putative lateral posterior nucleus.

Structure	Level	Pattern	Image	Note
TS22: peripeduncular nucleus	Present		4C	Putative peripeduncular nucleus is positive for Lhx2 expression.
TS22: medial geniculate nucleus	Strong	Regionally restricted	4C	Ventral subdivision of medial geniculate nucleus strongly expresses Lhx2.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Strong	Regionally restricted	4M	Band with high levels of Lhx2 expression located medial to band with high Lhx9 expression.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Strong	Regionally restricted	4N	Band with high levels of Lhx2 expression located medial to band with high Lhx9 expression.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	8A	Expression in lateral part of dorsal thalamus.