Probe:	Sfrp5 cDNA
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS21: mesonephros of female	Absent		1A female
TS21: ovary	Absent		1A female

Structure	Level	Pattern	Image	Note
TS21: mesonephros of male	Present	Regionally restricted	1A male	There is diffuse expression at the lateral edge.
TS21: coelomic epithelium of male mesonephros	Present		1A male
TS21: epithelium of paramesonephric duct of male	Present		1A male
TS21: mesenchyme of paramesonephric duct of male	Present		1A male
TS21: testis	Absent		1A male

Structure	Level	Pattern	Image	Note
TS21: mesonephros of female	Weak		1B female
TS21: ovary	Absent		1B female

Structure	Level	Pattern	Image	Note
TS21: epithelium of paramesonephric duct of male	Present		1B male
TS21: testis	Absent		1B male

Structure	Level	Pattern	Image	Note
TS21: mesonephros of female	Trace		1C	Expression was often found to be undetectable.
TS21: ovary	Absent		1C

Structure	Level	Pattern	Image	Note
TS21: epithelium of paramesonephric duct of male	Present		1D
TS21: testis	Absent		1D

Structure	Level	Pattern	Image	Note
TS22: epithelium of paramesonephric duct of female	Strong		1E female
TS22: paramesonephric duct of female	Strong		1E female
TS22: ovary	Absent		1E female

Structure	Level	Pattern	Image	Note
TS22: epithelium of paramesonephric duct of male	Present		1E male
TS22: testis	Absent		1E male

Structure	Level	Pattern	Image	Note
TS22: paramesonephric duct of female	Strong		1F female
TS22: ovary	Absent		1F female

Structure	Level	Pattern	Image	Note
TS22: epithelium of paramesonephric duct of male	Present		1F male
TS22: testis	Absent		1F male

Structure	Level	Pattern	Image	Note
TS21: mesonephros of male	Trace		3C px/px

Structure	Level	Pattern	Image	Note
TS21: mesonephros of male	Present	Regionally restricted	3C +/+	There is diffuse expression at the lateral edge with a broad domain of expression at the cranial end of the mesonephros and the absence of expression in the most caudal regions of the duct.