Gene Expression Data
Assay Details
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Assay
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Reference:
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J:60742
Rosenfeld ME, et al., Prevention of hepatic apoptosis and embryonic lethality in RelA/TNFR-1 double knockout mice. Am J Pathol. 2000 Mar;156(3):997-1007
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7523931
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Gene symbol:
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Nfkbia
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Gene name:
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nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha
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Antibody:
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IkBa antibody (C-21; sc-371)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Data not shown; presented in Table 1 and text.
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Results
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Specimen
wild type:
embryonic day 13.5
(more  )
Specimen
wild type:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: liver |
hepatoblast |
Moderate |
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| TS21: liver |
endothelial cell |
Present |
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High levels of expression were detected.
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| TS21: liver |
hematopoietic cell |
Present |
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High levels of expression were detected.
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| TS21: liver |
megakaryocyte |
Present |
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High levels of expression were detected.
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Specimen
RelA-/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Relatm1Bal/Relatm1Bal
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: liver |
endothelial cell |
Weak |
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Expression was decreased in the RelA-/- mutants.
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| TS21: liver |
hematopoietic cell |
Weak |
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Expression was decreased in the RelA-/- mutants.
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| TS21: liver |
hepatoblast |
Weak |
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Expression was decreased in the RelA-/- mutants.
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| TS21: liver |
megakaryocyte |
Weak |
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Expression was decreased in the RelA-/- mutants.
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Specimen
TNFR-1-/-:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Tnfrsf1atm1Imx/Tnfrsf1atm1Imx
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: liver |
hepatoblast |
Moderate |
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Expression was similar to wild type.
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| TS22: liver |
endothelial cell |
Present |
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Expression was similar to wild type.
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| TS22: liver |
hematopoietic cell |
Present |
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Expression was similar to wild type.
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| TS22: liver |
megakaryocyte |
Present |
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Expression was similar to wild type.
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