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Gene Expression Data
Assay Details
Assay
Reference: J:218555 Sheikh BN, et al., Excessive versus physiologically relevant levels of retinoic acid in embryonic stem cell differentiation. Stem Cells. 2014 Jun;32(6):1451-8
Assay type: RT-PCR
MGI Accession ID: MGI:7522527
Gene symbol: Hoxa4
Gene name: homeobox A4
Probe: Hoxa4 pF, Hoxa4 pR
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Pgk1 and Hsp90ab1.
Results Image: 1C Hoxa4
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
E6.5 E6.5 TS9: embryo Trace (a) 0.025 µg; total RNA Not Specified Not Specified
E7.5 E7.5 TS11: embryo Present (b) 0.08-0.1 µg; total RNA Not Specified Not Specified
E8.5 E8.5 TS13: embryo Strong 0.5 µg; total RNA Not Specified Not Specified
E10.5 E10.5 TS17: embryo Very strong (c) 1 µg; total RNA Not Specified Not Specified
Notes:
(a) Very low level.
(b) More than 8-fold increase in expression over E6.5.
(c) Significantly higher compared with younger embryos.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory