Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:215514
Saulnier A, et al., The doublesex homolog Dmrt5 is required for the development of the caudomedial cerebral cortex in mammals. Cereb Cortex. 2013 Nov;23(11):2552-67
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7522294
|
|
Gene symbol:
|
Calb2
|
|
Gene name:
|
calbindin 2
|
|
Antibody:
|
anti-Calretinin (AB5054)
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
|
Results
|
Specimen
S7B rostral Dmrt5+/+:
embryonic day 15.5
(more  )
Specimen
S7B rostral Dmrt5<+/+>:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S7B rostral Dmrt5<-/->:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Cajal-Retzius cell |
Weak |
Regionally restricted |
S7B rostral right
|
A few positive cells remained in the most medial portion of the mutant cortex. Some immunopositive cells also accumulated in the form of aggregates at the border between the neocortex and the piriform cortex.
|
Specimen
S7B caudal Dmrt5+/+:
embryonic day 15.5
(more )
Specimen
S7B caudal Dmrt5<+/+>:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S7B caudal Dmrt5<-/->:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Cajal-Retzius cell |
Weak |
Regionally restricted |
S7B caudal right
|
A few positive cells remained in the most medial portion of the mutant cortex.
|
| TS23: cortical subplate |
|
Weak |
|
S7B caudal right
|
|
|
Analysis Tools
