Gene Expression Data
Assay Details
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Assay
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Reference:
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J:215514
Saulnier A, et al., The doublesex homolog Dmrt5 is required for the development of the caudomedial cerebral cortex in mammals. Cereb Cortex. 2013 Nov;23(11):2552-67
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7522146
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Gene symbol:
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Nefm
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Gene name:
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neurofilament, medium polypeptide
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Results
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Specimen
S3B Rostral Dmrt5+/+:
embryonic day 18.5
(more  )
Specimen
S3B Rostral Dmrt5<+/+>:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
Present |
Regionally restricted |
S3B Rostral Dmrt5+/+
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Expression was detected in corticofugal axons extending toward the subpallium.
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Specimen
S3B Rostral Dmrt5<-/->:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
Present |
Regionally restricted |
S3B Rostral Dmrt5-/-
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Defects in axonal connections were noted in the mutants.
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Specimen
S3B Dmrt5+/+ middle:
embryonic day 18.5
(more )
Specimen
S3B Dmrt5<+/+> middle:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral internal capsule |
Present |
Regionally restricted |
S3B Dmrt5+/+
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Expression was detected in a large fan-shaped axonal bundle.
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| TS26: forebrain |
Present |
Regionally restricted |
S3B Dmrt5+/+
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Many thalamocortical axons invade the cortex
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Specimen
S3B Dmrt5<-/-> middle:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
Present |
Regionally restricted |
S3B Dmrt5-/-
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Defects in axonal connections were noted in the mutants. Fewer thalamocortical axons invade the cortex and aberrant axons extend downwards.
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Specimen
S3B Caudal Dmrt5+/+:
embryonic day 18.5
(more )
Specimen
S3B Caudal Dmrt5<+/+>:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
Present |
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S3B Caudal Dmrt5+/+
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Specimen
S3B Caudal Dmrt5<-/->:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
Present |
Regionally restricted |
S3B Caudal Dmrt5-/-
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Defects in axonal connections were noted in the mutants. An aberrant axon bundle was detected near the junction of the diencephalon and basal telencephalon.
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Specimen
S3C Dmrt5+/+:
embryonic day 18.5
(more )
Specimen
S3C Dmrt5<+/+>:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
Present |
Regionally restricted |
S3C Dmrt5+/+
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Immunoreactive thalamocortical axons pass through the striatum into the cortex in the wild type brain.
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Specimen
S3C Dmrt5<-/->:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
Present |
Regionally restricted |
S3C Dmrt5-/-
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Defects in axonal connections were noted in the mutants. Thalamocortical axons did not enter the cortex in the mutants.
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Analysis Tools