Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:215514
Saulnier A, et al., The doublesex homolog Dmrt5 is required for the development of the caudomedial cerebral cortex in mammals. Cereb Cortex. 2013 Nov;23(11):2552-67
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7522074
|
|
Gene symbol:
|
Auts2
|
|
Gene name:
|
autism susceptibility candidate 2
|
|
Probe:
|
Auts2 cDNA
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
7E Dmrt5+/+:
embryonic day 18.5
(more  )
Specimen
7E Dmrt5<+/+>:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Strong |
Graded |
7E Dmrt5+/+
|
Strong graded expression across the neocortex.
|
| TS26: cortical plate |
Present |
Graded |
7E Dmrt5+/+
|
Broadly expressed in the cortical plate in a high-rostral to low-caudal gradient.
|
Specimen
7E Dmrt5<-/->:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
7E Dmrt5-/-
|
Expression appeared to expand to almost the caudal end of the cortex.
|
|
Analysis Tools
