Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:215514
Saulnier A, et al., The doublesex homolog Dmrt5 is required for the development of the caudomedial cerebral cortex in mammals. Cereb Cortex. 2013 Nov;23(11):2552-67
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7522065
|
|
Gene symbol:
|
Epha7
|
|
Gene name:
|
Eph receptor A7
|
|
Probe:
|
Epha7 cDNA3
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
7B Dmrt5+/+:
embryonic day 18.5
(more  )
Specimen
7B Dmrt5<+/+>:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: frontal cortex |
Present |
Regionally restricted |
7B Dmrt5+/+
|
High expression was detected in the motor cortex region.
|
| TS26: parietal cortex |
Weak |
Regionally restricted |
7B Dmrt5+/+
|
Low expression was centered on the future somatosensory area.
|
Specimen
7B Dmrt5<-/->:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
7B Dmrt5-/-
|
The high expression domain of the mutant appeared to occupy most of the reduced cortex. The low expression domain was reduced and shifted caudally.
|
|
Analysis Tools
