Gene Expression Data
Assay Details
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Assay
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Reference:
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J:215514
Saulnier A, et al., The doublesex homolog Dmrt5 is required for the development of the caudomedial cerebral cortex in mammals. Cereb Cortex. 2013 Nov;23(11):2552-67
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7522017
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Gene symbol:
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Lhx2
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Gene name:
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LIM homeobox protein 2
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Probe:
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Lhx2 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5B sagittal Dmrt5+/+:
embryonic day 12.5
(more  )
Specimen
5B sagittal Dmrt5<+/+>:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5B sagittal Dmrt5<-/->:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cerebral cortex |
Weak |
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5B Dmrt5-/- top
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Expression was lower in the mutant compared to control and no longer demonstrated a gradient.
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Specimen
5B coronal Dmrt5+/+:
embryonic day 12.5
(more )
Specimen
5B coronal Dmrt5<+/+>:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5B coronal Dmrt5<-/->:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cerebral cortex |
Weak |
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5B Dmrt5-/- bottom
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Expression was lower in the mutant compared to control and no longer demonstrated a gradient.
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Specimen
not shown Dmrt5+/+:
embryonic day 9.5
(more )
Specimen
not shown Dmrt5<+/+>:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown Dmrt5<-/->:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: telencephalon |
Present |
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Staining in the mutant appeared similar to control embryos.
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Specimen
S8A rostral Dmrt5+/+:
embryonic day 10.5
(more )
Specimen
S8A rostral Dmrt5<+/+>:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S8A rostral Dmrt5<-/->:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Present |
Regionally restricted |
S8A right
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Staining appeared slightly decreased and the negative domain of expression in the dorsal midline was reduced in the mutant compared to control.
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Specimen
S8B caudal Dmrt5+/+:
embryonic day 10.5
(more )
Specimen
S8B caudal Dmrt5<+/+>:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S8B caudal Dmrt5<-/->:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Present |
Regionally restricted |
S8B right
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Staining appeared slightly decreased and the negative domain of expression in the dorsal midline was reduced in the mutant compared to control.
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Specimen
S10A Dmrt5+/+:
embryonic day 18.5
(more )
Specimen
S10A Dmrt5<+/+>:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
S10A Lhx2 Dmrt5+/+
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Layer II-III specific neocortical marker.
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Specimen
S10A Dmrt5<-/->:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
S10A Lhx2 Dmrt5-/-
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In the mutant, expression appeared in a pial to ventricular order similar to wild type. The ventral border of expression was shifted medially.
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Specimen
S10B Dmrt5+/+:
embryonic day 18.5
(more )
Specimen
S10B Dmrt5<+/+>:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
S10B Lhx2 Dmrt5+/+
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Layer II-III specific neocortical marker.
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Specimen
S10B Dmrt5<-/->:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
S10B Lhx2 Dmrt5-/-
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In the mutant, expression appeared in a pial to ventricular order similar to wild type.
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Analysis Tools