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Gene Expression Data
Assay Details
Assay
Reference: J:263975 Xie Z, et al., A Golgi Lipid Signaling Pathway Controls Apical Golgi Distribution and Cell Polarity during Neurogenesis. Dev Cell. 2018 Mar 26;44(6):725-740.e4
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7520522
Gene symbol: Eomes
Gene name: eomesodermin
Antibody: anti-TBR2/Eomes [EPR19012] (ab183661)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Cy2
Results
Specimen S1B Tbr2 Pax6: embryonic day 12.5 (more )
Note: Double labeled: green - Eomes; red - Pax6 (assay MGI:7520521).
Structure Level Pattern Image Note
TS20: neocortex Present S1B Tbr2 , S1B Merge
Specimen S2G Tbr2 Control: embryonic day 14.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls. Double labeled: green - Eomes; red - Pax6 (assay MGI:7520521).
Structure Level Pattern Image Note
TS22: cerebral cortex subventricular zone Present S2G Control (merged) , S2G Control Tbr2
Specimen S2G Tbr2 DKO: embryonic day 14.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre. Double labeled: green - Eomes; red - Pax6 (assay MGI:7520521).
Structure Level Pattern Image Note
TS22: neocortex Present Regionally restricted S2G DKO (merged) , S2G DKO Tbr2 Neocortical layers not distinguishable.
Specimen S2H Tbr2 Control: embryonic day 13.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls. Double labeled: green - Eomes; red - Pax6 (assay MGI:7520521).
Structure Level Pattern Image Note
TS21: cerebral cortex subventricular zone Present S2H Control Tbr2 , S2H Control (merged)
Specimen S2H Tbr2 DKO: embryonic day 13.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre. Double labeled: green - Eomes; red - Pax6 (assay MGI:7520521).
Structure Level Pattern Image Note
TS21: cerebral cortex subventricular zone Present Regionally restricted S2H DKO (merged) , S2H DKO Tbr2 Neocortical layers not completely disrupted - largely preserved at E13.5
Specimen S3D Tbr2 Control: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls. Double labeled: green - Eomes; red - Pax6 (assay MGI:7520521).
Structure Level Pattern Image Note
TS20: neocortex Present S3D Control (merged)
Specimen S3D Tbr2 DKO: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre. Double labeled: green - Eomes; red - Pax6 (assay MGI:7520521).
Structure Level Pattern Image Note
TS20: neocortex Present S3D DKO (merged) Frequency and distribution of expressing cells appeared similar to control.
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06/12/2024
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