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Gene Expression Data
Assay Details
Assay
Reference: J:263975 Xie Z, et al., A Golgi Lipid Signaling Pathway Controls Apical Golgi Distribution and Cell Polarity during Neurogenesis. Dev Cell. 2018 Mar 26;44(6):725-740.e4
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7520521
Gene symbol: Pax6
Gene name: paired box 6
Antibody: anti PAX6 (DSHB)
Detection system: Biotinylated secondary antibody/[Strept]avidin
Results
Specimen S1B Pax6: embryonic day 12.5 (more )
Note: Double labeled: red - Pax6; green - Eomes (assay MGI:7520522).
Structure Level Pattern Image Note
TS20: neocortex Present Widespread S1B Pax6 , S1B Merge Expression in neural stem cells occupying nearly entire neocortex.
Specimen S2G Pax6 Control: embryonic day 14.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls. Double labeled: red - Pax6; green - Eomes (assay MGI:7520522).
Structure Level Pattern Image Note
TS22: cerebral cortex ventricular layer Present S2G Control Pax6 , S2G Control (merged)
Specimen S2G Pax6 DKO: embryonic day 14.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre. Double labeled: red - Pax6; green - Eomes (assay MGI:7520522).
Structure Level Pattern Image Note
TS22: neocortex Present Regionally restricted S2G DKO (merged) , S2G DKO Pax6 Neocortical layers not distinguishable.
Specimen S2H Pax6 Control: embryonic day 13.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls. Double labeled: red - Pax6; green - Eomes (assay MGI:7520522).
Structure Level Pattern Image Note
TS21: cerebral cortex ventricular layer Present S2H Control Pax6 , S2H Control (merged)
Specimen S2H Pax6 DKO: embryonic day 13.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre. Double labeled: red - Pax6; green - Eomes (assay MGI:7520522).
Structure Level Pattern Image Note
TS21: cerebral cortex ventricular layer Present Regionally restricted S2H DKO Pax6 , S2H DKO (merged) Neocortical layers not completely disrupted - largely preserved at E13.5
Specimen S3D Pax6 Control: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls. Double labeled: red - Pax6; green - Eomes (assay MGI:7520522).
Structure Level Pattern Image Note
TS20: neocortex Present S3D Control (merged)
Specimen S3D Pax DKO: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre. Double labeled: red - Pax6; green - Eomes (assay MGI:7520522).
Structure Level Pattern Image Note
TS20: neocortex Present S3D DKO (merged) Frequency and distribution of expressing cells appeared similar to that of control.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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