Gene Expression Data
Assay Details
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Assay
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Reference:
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J:263975
Xie Z, et al., A Golgi Lipid Signaling Pathway Controls Apical Golgi Distribution and Cell Polarity during Neurogenesis. Dev Cell. 2018 Mar 26;44(6):725-740.e4
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7520513
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Gene symbol:
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Pitpnb
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Gene name:
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phosphatidylinositol transfer protein, beta
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Antibody:
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anti-PITPNB
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to Cy2
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Results
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Specimen
1B Control:
embryonic day 12.5
(more  )
Note: Wild-type littermate embryos were used as controls.
Specimen
1B Control:
(close  )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Wild-type littermate embryos were used as controls.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: neocortex |
Strong |
Widespread |
1B Control +DAPI
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1B Control
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Robust expression in cytoplasm of all neocortical cells.
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Specimen
1B PitpnB KO:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Pitpnbtm2.2Vab/Pitpnbtm2.2Vab
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: neocortex |
Trace |
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1B Pitpnb KO +DAPI
,
1B Pitpnb KO
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Expression strongly diminished compared to control.
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Analysis Tools