Gene Expression Data
Assay Details
Assay
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Reference:
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J:167262
Sumida H, et al., LPA4 regulates blood and lymphatic vessel formation during mouse embryogenesis. Blood. 2010 Dec 2;116(23):5060-70
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7520001
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Gene symbol:
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Acta2
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Gene name:
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actin alpha 2, smooth muscle, aorta
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Antibody:
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anti-alpha-SMA
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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Knock-outs were either female Lpar4<tm1Sati>/Lpar4<tm1Sati> or male Lpar4<tm1Sati>/Y. Both have been annotated.
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Results
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Specimen
4I:
embryonic day 18.5
(more )
Specimen
4I:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
4J Female:
(close )
Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Lpar4tm1Sati/Lpar4tm1Sati
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Age:
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embryonic day 18.5
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Sex:
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Female
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Type:
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section
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: blood vessel smooth muscle |
Present |
Regionally restricted |
4J
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Some of the dilated blood vessels in this mutant had were poorly covered with positive cells.
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Specimen
4J Male:
embryonic day 18.5; Lpar4tm1Sati/Y
(more )
Specimen
4J Male:
(close )
Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Lpar4tm1Sati/Y
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: blood vessel smooth muscle |
Present |
Regionally restricted |
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Some of the dilated blood vessels in this mutant had were poorly covered with positive cells.
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