Gene Expression Data
Assay Details
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Assay
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Reference:
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J:27992
Keeney DS, et al., Cholesterol side-chain cleavage cytochrome P450 gene expression in the primitive gut of the mouse embryo does not require steroidogenic factor 1. Mol Endocrinol. 1995 Aug;9(8):1091-8
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7517185
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Gene symbol:
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Cyp11a1
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Gene name:
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cytochrome P450, family 11, subfamily a, polypeptide 1
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Probe:
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P450scc probe1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1:
embryonic day 14.5
(more  )
Specimen
1:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: adrenal gland |
Present |
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1
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| TS22: hindgut |
Present |
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1
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| TS22: midgut |
Absent |
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1
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Expression was undetectable in the segment of midgut immediately ventral to the hindgut.
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| TS22: dermis |
Present |
Regionally restricted |
1
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Expression was detected in a subset of unidentified cells in the dermal mesenchyme of the skin; the cells were distributed among those tissues that will form superficial muscle layers and connective tissue elements of the dermis.
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Specimen
2A:
embryonic day 14.5
(more )
Specimen
2A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: hindgut |
intestinal epithelial cell |
Present |
Regionally restricted |
2C
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2A
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Expression was detected in hindgut luminal epithelial cells.
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Specimen
2D:
embryonic day 14.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: dermis |
Present |
Regionally restricted |
2D
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2F
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Expression was detected in the dermal mesenchyme. There were local concentrations of cells along the abdominal wall at the level of the pelvis, where the hindlimbs come off the body wall.
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Specimen
not shown:
embryonic day 17.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hindgut |
Weak |
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Specific signal was still detectable at E17.5 but at levels closer to background.
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| TS25: skin |
Weak |
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Specific signal was still detectable at E17.5 but at levels closer to background.
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Analysis Tools