Mtrr RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:312368 Imbard A, et al., Liver and brain differential expression of one-carbon metabolism genes during ontogenesis. Sci Rep. 2021 Oct 26;11(1):21132
Assay type:	RT-PCR
MGI Accession ID:	MGI:7516769
Gene symbol:	Mtrr
Gene name:	5-methyltetrahydrofolate-homocysteine methyltransferase reductase

Probe:	Mtrr-pF, Mtrr-pR
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Gapdh.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
E9	E9.0 (a)	TS14: hepatic primordium	Weak (b)	Not Specified; total RNA	OF1		Not Specified
E11	E11.0 (a)	TS18: liver	Weak	Not Specified; total RNA	OF1		Not Specified
E13	E13.0 (a)	TS21: liver	Weak	Not Specified; total RNA	OF1		Not Specified
E15	E15.0 (a)	TS23: liver	Weak	Not Specified; total RNA	OF1		Not Specified
E17	E17.0 (a)	TS25: liver	Trace (c)	Not Specified; total RNA	OF1		Not Specified
P0	P0	TS27: liver	Weak (d)	Not Specified; total RNA	OF1		Not Specified
P3	P3	TS27: liver	Weak	Not Specified; total RNA	OF1		Not Specified
P5	P5	TS28: liver	Weak	Not Specified; total RNA	OF1		Not Specified
P10	P10	TS28: liver	Weak	Not Specified; total RNA	OF1		Not Specified
P15	P15	TS28: liver	Weak	Not Specified; total RNA	OF1		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Relatively stable low level of expression between E9 and E15.
(c) Decreased expression at E17.
(d) Slight increase in expression between E17 and P1.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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