Antibody:	anti-Gata2
Detection system:	Secondary antibody coupled to Alexa Fluor 546
Assay notes:	Controls were either wild-type Gata2<c/c> or Gata2<c/+> littermates without Cre-transgene or wild-type and Gata2<c/+> littermates with Cre-transgene that all showed normal inner ear morphology during embryonic development.

Structure	Level	Pattern	Image	Note
TS22: vestibular component epithelium	Strong		3M
TS22: vestibular component mesenchyme	Strong	Regionally restricted	3M	Expression is in the mesenchyme surrounding the vestibular epithelium.

Structure	Level	Pattern	Image	Note
TS22: vestibular component epithelium	Absent		3O
TS22: vestibular component mesenchyme	Absent		3O

Structure	Cell Type	Level	Pattern	Image	Note
TS24: vestibular component epithelium		Present	Regionally restricted	5G	Expression is in the otic nonsensory epithelium and does not overlap with the dark cells.
TS24: utricle epithelium		Present		5G
TS24: semicircular canal mesenchyme		Present	Regionally restricted	5G	Expression is in the mesenchyme surrounding the utricle epithelium.
TS24: vestibular component epithelium	vestibular dark cell	Absent		5G

Structure	Level	Pattern	Image	Note
TS23: superior semicircular canal epithelium	Strong	Regionally restricted	6O	Expression is in the nonsensory epithelium.
TS23: inner ear mesenchyme	Present	Regionally restricted	6O	Expression is in the mesenchyme surrounding the duct.

Structure	Level	Pattern	Image	Note
TS26: crus commune	Present		6Q
TS26: otic capsule	Present	Regionally restricted	6Q	Expression is as a continuous membrane-like layer inside the condensed capsule.

Structure	Level	Pattern	Image	Note
TS26: cochlear duct epithelium	Present	Regionally restricted	6S	Expression is in the lateral duct.
TS26: cochlear duct mesenchyme	Present	Regionally restricted	6S	Expression is in the mesenchyme surrounding the duct.
TS26: otic capsule	Present	Regionally restricted	6S	Expression is as a continuous membrane-like layer inside the condensed capsule.