Gene Expression Data
Assay Details
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Assay
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Reference:
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J:267238
Sun K, et al., Hepatocyte expression of serum response factor is essential for liver function, hepatocyte proliferation and survival, and postnatal body growth in mice. Hepatology. 2009 May;49(5):1645-54
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7514929
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Gene symbol:
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Srf
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Gene name:
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serum response factor
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Results
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Specimen
1B:
embryonic day 11.5
(more  )
Specimen
1B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: liver |
cardiac muscle cell |
Strong |
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1B
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Higher expression in cardiac myocytes.
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Specimen
1C:
embryonic day 13.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: liver |
hepatoblast |
Weak |
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1C
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Lower expression within hepatoblasts.
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| TS21: liver sinusoid |
endothelial cell of hepatic sinusoid |
Present |
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1C
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Specimen
1D:
embryonic day 12.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: liver |
hematopoietic cell |
Present |
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1D
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Specimen
1E:
embryonic day 16.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: liver |
hepatoblast |
Strong |
Regionally restricted |
1E
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Increased expression in hepatoblasts, clearly localized within nucleus.
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| TS24: liver sinusoid |
endothelial cell of hepatic sinusoid |
Present |
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1E
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| TS24: hematopoietic system |
hematopoietic cell |
Present |
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1E
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Specimen
1F:
postnatal adult
(more )
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: liver |
hepatoblast |
Present |
Regionally restricted |
1F
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Expression in hepatoblasts, clearly localized within nucleus.
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| TS28: liver sinusoid |
endothelial cell of hepatic sinusoid |
Present |
Regionally restricted |
1F
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Specimen
1G:
postnatal adult
(more )
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3A:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6 * SJL
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Mutant Allele(s):
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Srftm1Rmn/Srf+
Tg(Alb1-cre)1Khk/0
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: liver |
hepatocyte |
Present |
Widespread |
3A
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Expression hepatocytes throughout parenchyma.
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| TS28: liver sinusoid |
endothelial cell of hepatic sinusoid |
Present |
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3A
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6 * SJL
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Mutant Allele(s):
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Srftm1Rmn/Srftm1Rmn
Tg(Alb1-cre)1Khk/0
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: liver |
hepatocyte |
Ambiguous |
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3B
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Expression undetectable in hepatocytes of a subset of livers.
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| TS28: liver sinusoid |
endothelial cell of hepatic sinusoid |
Present |
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3B
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Specimen
3C:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6 * SJL
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Mutant Allele(s):
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Srftm1Rmn/Srftm1Rmn
Tg(Alb1-cre)1Khk/0
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: liver |
hepatocyte |
Present |
Regionally restricted |
3C
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Majority displayed variability of expression with areas of expressing hepatocytes interspersed with areas of non-expessing hepatocytes.
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| TS28: liver sinusoid |
endothelial cell of hepatic sinusoid |
Present |
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3C
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Specimen
5A:
postnatal adult
(more )
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: liver |
hepatocyte |
Present |
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5A
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6 * SJL
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Mutant Allele(s):
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Srftm1Rmn/Srftm1Rmn
Tg(Alb1-cre)1Khk/0
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: liver |
hepatocyte |
Trace |
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5D
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In some cases, loss of expression in hepatocytes was extensive.
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Specimen
5G:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6 * SJL
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Mutant Allele(s):
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Srftm1Rmn/Srftm1Rmn
Tg(Alb1-cre)1Khk/0
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: liver |
hepatocyte |
Weak |
Regionally restricted |
5G
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In some cases, loss of expression in hepatocytes was mosaic.
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