Gene Expression Data
Assay Details
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Assay
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Reference:
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J:317244
Lee J, et al., Fetal hematopoietic stem cells express MFG-E8 during mouse embryogenesis. Exp Mol Med. 2015 Jul 24;47:e174
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7514399
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Gene symbol:
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Mfge8
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Gene name:
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milk fat globule EGF and factor V/VIII domain containing
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Antibody:
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anti-MFG-E8
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Detection system:
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Secondary antibody
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Results
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Specimen
1d/f/g:
embryonic day 10.5
(more  )
Note: Double labeled: green - Mfge8; red - Cd34 (assay MGI:7514400).
Specimen
1d/f/g:
(close  )
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Genetic Background:
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ICR
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Mfge8; red - Cd34 (assay MGI:7514400).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS17: blood |
hematopoietic stem cell |
Strong |
Regionally restricted |
1d
,
1f
,
1g
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Co-expressed with Cd34 in large cells with prominent nuclei in the yolk sac blood islands as aggregates.
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| TS17: yolk sac mesenchyme |
hematopoietic stem cell |
Strong |
Regionally restricted |
1d
,
1f
,
1g
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Co-expressed with Cd34 in large cells with prominent nuclei in the yolk sac blood islands as aggregates.
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Specimen
2b/d:
embryonic day 11.5
(more )
Note: Double labeled: green - Mfge8; red - Cd34 (assay MGI:7514400).
Specimen
2b/d:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Mfge8; red - Cd34 (assay MGI:7514400).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: blood |
hematopoietic stem cell |
Present |
Regionally restricted |
2b
,
2d
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Co-expressed with Cd34 in the dorsal aorta and cardinal veins region.
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| TS19: cardinal vein |
hematopoietic stem cell |
Present |
Regionally restricted |
2b
,
2d
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Co-expressed with Cd34 in the dorsal aorta and cardinal veins region.
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| TS19: dorsal aorta |
hematopoietic stem cell |
Present |
Regionally restricted |
2b
,
2d
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Co-expressed with Cd34 in the dorsal aorta and cardinal veins region.
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Specimen
2f/h:
embryonic day 11.5
(more )
Note: Double labeled: green - Mfge8; red - Cd34 (assay MGI:7514400).
Specimen
2f/h:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Paraffin
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|
Note:
|
Double labeled: green - Mfge8; red - Cd34 (assay MGI:7514400).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: blood |
hematopoietic stem cell |
Present |
Regionally restricted |
2f
,
2h
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Co-expressed with Cd34 in many prominent colonies of eosinophilic and nucleate cells over the entire fetal liver parenchyma.
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| TS19: liver parenchyma |
hematopoietic stem cell |
Present |
Regionally restricted |
2f
,
2h
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Co-expressed with Cd34 in many prominent colonies of eosinophilic and nucleate cells over the entire fetal liver parenchyma.
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Specimen
3b/d:
embryonic day 11.5
(more )
Note: Double labeled: green - Mfge8; red - Cd34 (assay MGI:7514400).
Specimen
3b/d:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
|
Double labeled: green - Mfge8; red - Cd34 (assay MGI:7514400).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: blood |
hematopoietic stem cell |
Present |
Regionally restricted |
3d
,
3b
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Co-expressed with Cd34 in a number of cells with large nuclei in large fetal blood vessels in the chorioallantoic mesenchyme.
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| TS19: chorionic plate |
hematopoietic stem cell |
Present |
Regionally restricted |
3d
,
3b
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Co-expressed with Cd34 in a number of cells with large nuclei in large fetal blood vessels in the chorioallantoic mesenchyme.
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| TS19: labyrinthine zone |
hematopoietic stem cell |
Present |
Regionally restricted |
3d
,
3b
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Co-expressed with Cd34 in a number of cells with large nuclei in large fetal blood vessels in the chorioallantoic mesenchyme.
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Specimen
5b:
postnatal adult
(more )
Note: Double labeled: green - Mfge8; red - Adgre1 (assay MGI:7514403).
Specimen
5b:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Mfge8; red - Adgre1 (assay MGI:7514403).
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Specimen
not shown:
postnatal adult
(more )
Specimen
not shown:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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