Gene Expression Data
Assay Details
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Assay
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Reference:
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J:164447
Lee WC, et al., Dact2 is expressed in the developing ureteric bud/collecting duct system of the kidney and controls morphogenetic behavior of collecting duct cells. Am J Physiol Renal Physiol. 2010 Oct;299(4):F740-51
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7514151
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Gene symbol:
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Dact2
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Gene name:
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dishevelled-binding antagonist of beta-catenin 2
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Probe:
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Dact2 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2C:
embryonic day 11.5
(more  )
Specimen
2C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: ureteric bud |
Present |
Regionally restricted |
2C
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Expression in ureteric bud/collecting duct system detectable as early as E11.5.
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| TS19: ureteric stalk |
Present |
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2C
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No difference between ureteric tips and stalk regions.
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| TS19: ureteric tip |
Present |
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2C
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No difference between ureteric tips and stalk regions.
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Specimen
2D:
embryonic day 12.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: primitive collecting duct |
Strong |
Regionally restricted |
2D
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Expression in ureteric bud/collecting duct system becomes stronger during development.
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| TS20: ureteric tip |
Present |
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2D
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No difference between ureteric tips and stalk regions.
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| TS20: ureteric trunk |
Present |
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2D
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No difference between ureteric tips and stalk regions.
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| TS20: ureter |
Weak |
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2D
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Expression diminished in ureter itself.
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Specimen
2E:
embryonic day 14.5
(more )
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: collecting duct |
Strong |
Regionally restricted |
2E
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Clear pattern of expression by E14.5.
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| TS22: ureteric tip |
Present |
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2E
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No difference between ureteric tips and stalk regions.
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| TS22: ureteric trunk |
Present |
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2E
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No difference between ureteric tips and stalk regions.
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| TS22: ureter |
Weak |
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2E
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Expression diminished in ureter itself.
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Analysis Tools