Gene Expression Data
Assay Details
Assay
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Reference:
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J:92062
Pratt T, et al., The winged helix transcription factor Foxg1 facilitates retinal ganglion cell axon crossing of the ventral midline in the mouse. Development. 2004 Aug;131(15):3773-84
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7514063
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Gene symbol:
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Efnb2
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Gene name:
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ephrin B2
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Results
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Specimen
5A:
embryonic day 13.5
(more )
Specimen
5A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: neural retina |
Present |
Graded |
5A
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Dorsolateral (high) to ventrolateral (low) gradient of expression, with strongest expression dorsolaterally.
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TS21: optic nerve |
Weak |
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5A
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Weak expression in optic nerve.
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Specimen
5B:
embryonic day 16.5
(more )
Specimen
5B:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 16.5
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Sex:
|
Not Specified
|
Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5C:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Foxg1tm1(cre)Skm/Foxg1tm1(cre)Skm
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: neural retina |
Present |
Graded |
5C
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Dorsal (high) to ventral (low) gradient of expression.
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Specimen
5D:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Foxg1tm1(cre)Skm/Foxg1tm1(cre)Skm
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Age:
|
embryonic day 16.5
|
Sex:
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Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: neural retina |
Present |
Graded |
5D
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Strongest expression in dorsolateral retina.
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Specimen
6A:
embryonic day 13.5
(more )
Specimen
6A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
Present |
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6A
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Expression is most widespread in more dorsal structures in developing forebrain and eye.
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TS21: eye |
Present |
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6A
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Expression is most widespread in more dorsal structures in developing forebrain and eye.
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TS21: hypothalamus |
Present |
Regionally restricted |
6A
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Expression in ventral hypothalamic midline.
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Specimen
6B:
embryonic day 13.5
(more )
Specimen
6B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: hypothalamus |
Present |
Regionally restricted |
6B
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Expression in ventral hypothalamic midline.
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Specimen
6C:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Foxg1tm1(cre)Skm/Foxg1tm1(cre)Skm
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Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
6D:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Foxg1tm1(cre)Skm/Foxg1tm1(cre)Skm
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: hypothalamus |
Present |
Regionally restricted |
6D
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Expression in hypothalamic midline.
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Specimen
6E:
embryonic day 16.5
(more )
Specimen
6E:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: optic chiasma |
Present |
Regionally restricted |
6E
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Expression at optic chiasm where expressing cells contact optic nerve.
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Specimen
6F:
embryonic day 16.5
(more )
Specimen
6F:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: optic chiasma |
Present |
Regionally restricted |
6F
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Expression at optic chiasm where expressing cells contact optic nerve.
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Specimen
6G:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Foxg1tm1(cre)Skm/Foxg1tm1(cre)Skm
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Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: optic chiasma |
Present |
Regionally restricted |
6G
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Expression persists at mutant chiasm where it contacts Ephb2-expressing axons.
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Specimen
6H:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Foxg1tm1(cre)Skm/Foxg1tm1(cre)Skm
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: optic chiasma |
Present |
Regionally restricted |
6H
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Expression persists at mutant chiasm where it contacts Ephb2-expressing axons.
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