Gene Expression Data
Assay Details
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Assay
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Reference:
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J:127123
Barker N, et al., Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature. 2007 Oct 25;449(7165):1003-7
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7509963
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Gene symbol:
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Lgr5
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Gene name:
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leucine rich repeat containing G protein coupled receptor 5
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Reporter:
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GFP
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Antibody:
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anti-GFP
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Detection system:
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Secondary antibody
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Results
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Specimen
4d:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lgr5tm1(cre/ERT2)Cle/Lgr5+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: small intestine crypt of lieberkuhn |
Present |
Regionally restricted |
4d
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Expression is restricted to the 6-8 slender cells sandwiched between the Paneth cells at the crypt base; these are referred to as cycling crypt base columnar (CBC) cells.
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Specimen
S1:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lgr5tm1(cre/ERT2)Cle/Lgr5+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: small intestine crypt of lieberkuhn |
Present |
Regionally restricted |
S1_GFP
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Staining of serial sections reveals the expressing cells (cycling crypt base columnar cells) are dividing.
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Analysis Tools
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