Mki67 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:212415 Kinzel B, et al., Functional roles of Lgr4 and Lgr5 in embryonic gut, kidney and skin development in mice. Dev Biol. 2014 Jun 15;390(2):181-90
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7509222
Gene symbol:	Mki67
Gene name:	antigen identified by monoclonal antibody Ki 67

Antibody:	anti-Ki67 (TEC-3; M7249)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Results

2C wt ©

Specimen 2C wt: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: intestine epithelium	Present		2C wt

2C Lgr5KO ©

Specimen 2C Lgr5KO: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: intestine epithelium	Present		2C Lgr5KO	Expression in this mutant is the same as in wild-type.

2C Lgr4KO ©

Specimen 2C Lgr4KO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: intestine epithelium	Weak		2C Lgr4KO	In this mutant there is an approximate 3-fold reduction in positive cells compared to wild-type.

2C Lgr4/5dKO ©

Specimen 2C Lgr4/5dKO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: intestine epithelium	Weak		2C Lgr4/5dKO	In this mutant there is an approximate 3-fold reduction in positive cells compared to wild-type.

4F wt ©

Specimen 4F wt: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: epidermis basal layer	Present		4F wt

4F Lgr5KO ©

Specimen 4F Lgr5KO: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: epidermis basal layer	Present		4F Lgr5KO	Expression in this mutant is the same as wild-type.

4F Lgr4KO ©

Specimen 4F Lgr4KO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: epidermis basal layer	Present		4F Lgr4KO	In this mutant there is a significant reduction in positive cells compared to wild-type.

4F Lgr4/5dKO ©

Specimen 4F Lgr4/5dKO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: epidermis basal layer	Present		4F Lgr4/5dKO	In this mutant there is a significant reduction in positive cells compared to wild-type.

5D wt ©

Specimen 5D wt: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present		5D wt

5D Lgr5KO ©

Specimen 5D Lgr5KO: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present		5D Lgr5KO	Expression in this mutant is similar to wild-type.

5D Lgr4KO ©

Specimen 5D Lgr4KO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present		5D Lgr4KO	Expression in this mutant is similar to wild-type.

5D Lgr4/5dKO ©

Specimen 5D Lgr4/5dKO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present		5D Lgr4/5dKO	In this mutant there is a significant reduction in positive cells compared to wild-type.

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