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Gene Expression Data
Assay Details
Assay
Reference: J:212415 Kinzel B, et al., Functional roles of Lgr4 and Lgr5 in embryonic gut, kidney and skin development in mice. Dev Biol. 2014 Jun 15;390(2):181-90
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7509222
Gene symbol: Mki67
Gene name: antigen identified by monoclonal antibody Ki 67
Antibody: anti-Ki67 (TEC-3; M7249)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen 2C wt: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: intestine epithelium Present 2C wt

Specimen 2C Lgr5KO: embryonic day 16.5; Lgr5tm1.1(cre/ERT2)Npa/Lgr5tm1.1(cre/ERT2)Npa (more )
Structure Level Pattern Image Note
TS24: intestine epithelium Present 2C Lgr5KO Expression in this mutant is the same as in wild-type.

Specimen 2C Lgr4KO: embryonic day 16.5; Lgr4tm1.1(cre/ERT2)Npa/Lgr4tm1.1(cre/ERT2)Npa (more )
Structure Level Pattern Image Note
TS24: intestine epithelium Weak 2C Lgr4KO In this mutant there is an approximate 3-fold reduction in positive cells compared to wild-type.

Structure Level Pattern Image Note
TS24: intestine epithelium Weak 2C Lgr4/5dKO In this mutant there is an approximate 3-fold reduction in positive cells compared to wild-type.

Specimen 4F wt: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: epidermis basal layer Present 4F wt

Specimen 4F Lgr5KO: embryonic day 16.5; Lgr5tm1.1(cre/ERT2)Npa/Lgr5tm1.1(cre/ERT2)Npa (more )
Structure Level Pattern Image Note
TS24: epidermis basal layer Present 4F Lgr5KO Expression in this mutant is the same as wild-type.

Specimen 4F Lgr4KO: embryonic day 16.5; Lgr4tm1.1(cre/ERT2)Npa/Lgr4tm1.1(cre/ERT2)Npa (more )
Structure Level Pattern Image Note
TS24: epidermis basal layer Present 4F Lgr4KO In this mutant there is a significant reduction in positive cells compared to wild-type.

Structure Level Pattern Image Note
TS24: epidermis basal layer Present 4F Lgr4/5dKO In this mutant there is a significant reduction in positive cells compared to wild-type.

Specimen 5D wt: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: metanephros Present 5D wt

Specimen 5D Lgr5KO: embryonic day 16.5; Lgr5tm1.1(cre/ERT2)Npa/Lgr5tm1.1(cre/ERT2)Npa (more )
Structure Level Pattern Image Note
TS24: metanephros Present 5D Lgr5KO Expression in this mutant is similar to wild-type.

Specimen 5D Lgr4KO: embryonic day 16.5; Lgr4tm1.1(cre/ERT2)Npa/Lgr4tm1.1(cre/ERT2)Npa (more )
Structure Level Pattern Image Note
TS24: metanephros Present 5D Lgr4KO Expression in this mutant is similar to wild-type.

Structure Level Pattern Image Note
TS24: metanephros Present 5D Lgr4/5dKO In this mutant there is a significant reduction in positive cells compared to wild-type.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory