Lgr5 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:212415 Kinzel B, et al., Functional roles of Lgr4 and Lgr5 in embryonic gut, kidney and skin development in mice. Dev Biol. 2014 Jun 15;390(2):181-90
Assay type:	RNA in situ
MGI Accession ID:	MGI:7509159
Gene symbol:	Lgr5
Gene name:	leucine rich repeat containing G protein coupled receptor 5

Probe:	Lgr5 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

4H Lgr5 wt ©

Specimen 4H wt: embryonic day 16.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: hair follicle		Present		4H Lgr5 wt
TS24: dermis	fibroblast of dermis	Present	Regionally restricted	4H Lgr5 wt	Expression is in dermal fibroblasts.

4H Lgr5 Lgr5KO ©

Specimen 4H Lgr5KO: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: dermis	Absent		4H Lgr5 Lgr5KO
TS24: hair follicle	Absent		4H Lgr5 Lgr5KO

4H Lgr5 Lgr4KO ©

Specimen 4H Lgr4KO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: hair follicle		Present		4H Lgr5 Lgr4KO	Expression in this mutant was the same as in wild-type.
TS24: dermis	fibroblast of dermis	Present	Regionally restricted	4H Lgr5 Lgr4KO	Expression is in dermal fibroblasts. Expression in this mutant was the same as in wild-type.

4H Lgr5 Lgr4/5dKO ©

Specimen 4H Lgr4/5dKO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: dermis	Absent		4H Lgr5 Lgr4/5dKO
TS24: epidermis basal layer	Absent		4H Lgr5 Lgr4/5dKO

5F wt ©

Specimen 5F wt: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: renal tubule	Present	Regionally restricted	5F wt	Expression is in tubular epithelium.

5F Lgr5KO ©

Specimen 5F Lgr5KO: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: metanephros	Absent		5F Lgr5KO

5F Lgr4KO ©

Specimen 5F Lgr4KO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: renal tubule	Present	Regionally restricted	5F Lgr4KO	in tubular epithelium.

5F Lgr4/5dKO ©

Specimen 5F Lgr4/5dKO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: metanephros	Absent		5F Lgr4/5dKO

S2_Lgr5 wt ©

Specimen S2 wt: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: intestine	Present		S2_Lgr5 wt

S2_Lgr5 Lgr5KO ©

Specimen S2 Lgr5KO: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: intestine	Absent		S2_Lgr5 Lgr5KO

S2_Lgr5 Lgr4KO ©

Specimen S2 Lgr4KO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: intestine	Absent		S2_Lgr5 Lgr4KO

S2_Lgr5 Lgr4/5dKO ©

Specimen S2 Lgr4/5dKO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: intestine	Absent		S2_Lgr5 Lgr4/5dKO

S3_Lgr5 wt ©

Specimen S3 wt: embryonic day 16.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: hair follicle		Present		S3_Lgr5 wt
TS24: dermis	fibroblast of dermis	Present	Regionally restricted	S3_Lgr5 wt	Expression is in dermal fibroblasts.

S3_Lgr5 Lgr5KO ©

Specimen S3 Lgr5KO: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: dermis	Absent		S3_Lgr5 Lgr5KO
TS24: hair follicle	Absent		S3_Lgr5 Lgr5KO

S3_Lgr5 Lgr4KO ©

Specimen S3 Lgr4KO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: hair follicle		Present		S3_Lgr5 Lgr4KO	Expression in this mutant was the same as in wild-type.
TS24: dermis	fibroblast of dermis	Present	Regionally restricted	S3_Lgr5 Lgr4KO	Expression is in dermal fibroblasts. Expression in this mutant was the same as in wild-type.

Specimen S3 Lgr4/5dKO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: dermis	Absent		S3_Lgr5 Lgr4/5dKO
TS24: hair follicle	Absent		S3_Lgr5 Lgr4/5dKO

S4_Lgr5 wt ©

Specimen S4 wt: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: renal tubule	Present		S4_Lgr5 wt
TS24: Bowman's capsule of maturing renal corpuscle	Absent		S4_Lgr5 wt

Specimen S4 Lgr5KO: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: metanephros	Absent		S4_Lgr5 Lgr5KO

Specimen S4 Lgr4KO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: renal tubule	Present		S4_Lgr5 Lgr4KO
TS24: Bowman's capsule of maturing renal corpuscle	Absent		S4_Lgr5 Lgr4KO

Specimen S4 Lgr4/5dKO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: metanephros	Absent		S4_Lgr5 Lgr4/5dKO

S6B wt ©

Specimen S6 wt: embryonic day 16.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: hair follicle		Present		S6B wt
TS24: dermis	fibroblast of dermis	Present	Regionally restricted	S6B wt	Expression is in dermal fibroblasts.

S6B Lgr4KO ©

Specimen S6 Lgr4KO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: hair follicle		Present		S6B Lgr4KO	Expression in this mutant was the same as in wild-type.
TS24: dermis	fibroblast of dermis	Present	Regionally restricted	S6B Lgr4KO	Expression is in dermal fibroblasts. Expression in this mutant was the same as in wild-type.

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