Pparg RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:186530 Abbott BD, et al., Effects of perfluorooctanoic acid (PFOA) on expression of peroxisome proliferator-activated receptors (PPAR) and nuclear receptor-regulated genes in fetal and postnatal CD-1 mouse tissues. Reprod Toxicol. 2012 Jul;33(4):491-505
Assay type:	RT-PCR
MGI Accession ID:	MGI:7509143
Gene symbol:	Pparg
Gene name:	peroxisome proliferator activated receptor gamma

Probe:	Mm00440945_m1
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Actb.

Results

Image: 8_PPARgamma RNA

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
GD17 control	E17.5 (a)	TS25: spleen	Present	Not Specified; total RNA	CD-1		Not Specified	spleen from E17.5 embryo
PND1 control	P1	TS27: spleen	Present	Not Specified; total RNA	CD-1		Not Specified
PND7 control	P7	TS28: spleen	Present	Not Specified; total RNA	CD-1		Not Specified
PND14 control	P14	TS28: spleen	Present	Not Specified; total RNA	CD-1		Not Specified
PND21 control	P21	TS28: spleen	Present	Not Specified; total RNA	CD-1		Not Specified
PND28 control	P28	TS28: spleen	Present	Not Specified; total RNA	CD-1		Not Specified

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Increased expression from PND1 to 7, and then remained the same until PND28. Expressed at higher levels than Ppara or Ppard.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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