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Gene Expression Data
Assay Details
Assay
Reference: J:276652 Fang Q, et al., Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function. Sci Rep. 2019 Mar 4;9(1):3357
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7506977
Gene symbol: Limk2
Gene name: LIM domain kinase 2
Antibody: anti-LIMK2 (sc-5577)
Assay notes: Fixation with 4% polyoxymethylene.
Results
Specimen 1a Limk2 P21: postnatal day 21 (more )
Note: Double labeled: red - Limk2; green - Myo7a (assay MGI:7506974).
Structure Cell Type Level Pattern Image Note
TS28: cochlea epithelium cochlea auditory hair cell Present 1a LIMK2 P21 , 1a P21 Merged (right) Expression in nuclei of hair cells and supporting cells. No difference in expression from apical to basal turns.
TS28: cochlea epithelium supporting cell of cochlea Present 1a LIMK2 P21 , 1a P21 Merged (right) Expression in nuclei of hair cells and supporting cells. No difference in expression from apical to basal turns.
Specimen 1a Limk2 P30: postnatal day 30 (more )
Note: Double labeled: red - Limk2; green - Myo7a (assay MGI:7506974).
Structure Cell Type Level Pattern Image Note
TS28: cochlea epithelium cochlea auditory hair cell Present 1a P30 Merged (right) , 1a P30 z-stack (right) , 1a LIMK2 P30 Expression in nuclei of hair cells and supporting cells. No difference in expression from apical to basal turns.
TS28: cochlea epithelium supporting cell of cochlea Present 1a P30 Merged (right) , 1a P30 z-stack (right) , 1a LIMK2 P30 Expression in nuclei of hair cells and supporting cells. No difference in expression from apical to basal turns.
Specimen S2 Limk2 P21: postnatal day 21 (more )
Note: Double labeled: red - Limk2; blue - Sox2 (assay MGI:7506978).
Structure Cell Type Level Pattern Image Note
TS28: cochlea Deiter's cell Present S2_P21 Merged (right) , S2_P21 z-stack (right) , S2_LIMK2 P21
TS28: cochlea pillar cell Present S2_P21 Merged (right) , S2_P21 z-stack (right) , S2_LIMK2 P21
Specimen S2 LImk2 P30: postnatal day 30 (more )
Note: Double labeled: red - Limk2; blue - Sox2 (assay MGI:7506978).
Structure Cell Type Level Pattern Image Note
TS28: cochlea Deiter's cell Present S2_LIMK2 P30 , S2_P30 z-stack (right) , S2_P30 Merged (right)
TS28: cochlea pillar cell Present S2_LIMK2 P30 , S2_P30 z-stack (right) , S2_P30 Merged (right)
Specimen 2a Limk2 Cytoplasm WT: postnatal day 3 (more )
Note: Double labeled: red - Limk2; green - Myo7a (assay MGI:7506974).
Structure Cell Type Level Pattern Image Note
TS27: cochlea epithelium cochlea auditory hair cell Present 2a Cytoplasm WT Merged (right) , 2a Cytoplasm WT LIMK2 Expression mainly cytoplasmic at P3.
Specimen 2a Limk2 Cytoplasm DKO: postnatal day 3; Limk1tm1Zpj/Limk1tm1Zpj, Limk2tm1.1Tnak/Limk2tm1.1Tnak (more )
Note: Double labeled: red - Limk2; green - Myo7a (assay MGI:7506974).
Structure Level Pattern Image Note
TS27: cochlea epithelium Absent 2a Cytoplasm DKO LIMK2 , 2a Cytoplasm DKO Merged (right)
Specimen 2a Limk2 Nucleus WT: postnatal day 3 (more )
Note: Double labeled: red - Limk2; green - Myo7a (assay MGI:7506974).
Structure Level Pattern Image Note
TS27: cochlea epithelium Ambiguous 2a Nucleus WT Merged (right) , 2a Nucleus WT LIMK2
Specimen 2a Limk2 Nucleus DKO: postnatal day 3; Limk1tm1Zpj/Limk1tm1Zpj, Limk2tm1.1Tnak/Limk2tm1.1Tnak (more )
Note: Double labeled: red - Limk2; green - Myo7a (assay MGI:7506974).
Structure Level Pattern Image Note
TS27: cochlea epithelium Absent 2a Nucleus DKO Merged (right) , 2a Nucleus DKO LIMK2
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
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