Pals1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:191149 Alves CH, et al., Loss of CRB2 in the mouse retina mimics human retinitis pigmentosa due to mutations in the CRB1 gene. Hum Mol Genet. 2013 Jan 1;22(1):35-50
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7506777
Gene symbol:	Pals1
Gene name:	protein associated with LIN7 1, MAGUK family member

Antibody:	anti-MPP5 (17710-1-AP)
Detection system:	Secondary antibody coupled to Cy3

Results

Specimen 6C: embryonic day 18.5; Crb2^tm1.1Wij/? (more

)
Note: Controls could be Crb2^F/F or Crb2^F/+/Chx10Cre^+/-.

Structure	Level	Pattern	Image	Note
TS26: retina	Present	Regionally restricted	6C	Expression was detected in the subapical region.

Specimen 6D: embryonic day 18.5; Crb2^tm1.1Wij/Crb2^tm1.1Wij, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS26: retina	Present	Regionally restricted	6D	Staining in the mutant showed disruption at the subapical region.

Specimen 6I: postnatal month 3; Crb2^tm1.1Wij/? (more

)
Note: Controls could be Crb2^F/F or Crb2^F/+/Chx10Cre^+/-.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	6I	Expression was detected in the subapical region adjacent to the outer limiting membrane.

Specimen 6J: postnatal month 3; Crb2^tm1.1Wij/Crb2^tm1.1Wij, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	6J	Staining in the mutant showed disruption at the subapical region.

Specimen S1C: embryonic day 11.5; Crb2^tm1.1Wij/? (more

)
Note: Controls could be Crb2^F/F or Crb2^F/+/Chx10Cre^+/-.

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Present	Regionally restricted	S1C	Expression was detected in the subapical region of the neuroepithelial layer.

Specimen S1F: embryonic day 11.5; Crb2^tm1.1Wij/Crb2^tm1.1Wij, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Present	Regionally restricted	S1I , S1F	Expression was detected in the subapical region of the neuroepithelial layer.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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