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Gene Expression Data
Assay Details
Assay
Reference: J:282806 Chen NY, et al., An absence of lamin B1 in migrating neurons causes nuclear membrane ruptures and cell death. Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25870-25879
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7506222
Gene symbol: Lmnb2
Gene name: lamin B2
Antibody: anti-Lamin B2 [EPR9701]
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Two Lmnb2 antibodies were described, but the one used in this assay was not detailed. Anti-Lamin MGI:7506219 may have been used in this assay.
Results
Specimen S4A Control CP: embryonic day 18.5 (more )
Note: Double labeled: magenta - Lmnb2; white - recombinase reporter.
Structure Cell Type Level Pattern Image Note
TS26: cortical plate cerebral cortex neuron Present Regionally restricted S4A Control CP Merge , S4A Control CP Lamin B2 Expression distributed evenly along nuclear rim.
Specimen S4A Lmnb1 KO CP: embryonic day 18.5; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-tdTomato*,-EGFP*)Ees/Gt(ROSA)26Sortm1(CAG-tdTomato*,-EGFP*)Ees, Lmnb1tm1.1Sgy/Lmnb1tm1.1Sgy (more )
Note: Conditional mutant. Double labeled: magenta - Lmnb2; white - recombinase reporter.
Structure Cell Type Level Pattern Image Note
TS26: cortical plate cerebral cortex neuron Present Regionally restricted S4A Lmnb1 KO CP Lamin B2 , S4A Lmnb1 KO CP Merge Expression distributed asymmetrically.
Specimen S4A Lmnb1 KO VZ: embryonic day 18.5; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-tdTomato*,-EGFP*)Ees/Gt(ROSA)26Sortm1(CAG-tdTomato*,-EGFP*)Ees, Lmnb1tm1.1Sgy/Lmnb1tm1.1Sgy (more )
Note: Conditional mutant. Double labeled: magenta - Lmnb2; white - recombinase reporter.
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex ventricular zone cerebral cortex neuron Present Regionally restricted S4A Lmnb1 KO VZ Merge , S4A Lmnb1 KO VZ Lamin B2 Expression distributed evenly.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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