Gene Expression Data
Assay Details
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Assay
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Reference:
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J:320752
Lu YC, et al., Small-conductance calcium-activated K(+) channels 3 (SK3) regulate blastocyst hatching by control of intracellular calcium concentration. Hum Reprod. 2012 May;27(5):1421-30
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7495872
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Gene symbol:
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Kcnn3
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Gene name:
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potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3
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Antibody:
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Anti-SK3 (AB5350)
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
1E Zygote:
embryonic day 0.5
(more  )
Specimen
1E Zygote:
(close  )
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Genetic Background:
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ICR
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1E 2-cell:
embryonic day 1.0
(more )
Specimen
1E 2-cell:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
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1E Merge 2-cell
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1E SK3 2-cell
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Expression was detected in the cytoplasm but not in the membrane of the embryo.
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Specimen
1E 4-cell:
embryonic day 2.0
(more )
Specimen
1E 4-cell:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-cell stage embryo |
Present |
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1E SK3 4-cell
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1E Merge 4-cell
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Expression was detected in the cytoplasm but not in the membrane of the embryo.
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Specimen
1E 8-cell:
embryonic day 2.5
(more )
Specimen
1E 8-cell:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Present |
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1E SK3 8-cell
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1E Merge 8-cell
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Expression was detected in the cytoplasm but not in the membrane of the embryo.
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Specimen
1E Morula:
embryonic day 3.0
(more )
Specimen
1E Morula:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
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1E Merge Morula
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1E SK3 Morula
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Expression was detected in the cytoplasm but not in the membrane of the embryo.
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Specimen
1E Blastocyst:
embryonic day 3.5
(more )
Note: expanded blastocyst stage
Specimen
1E Blastocyst:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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expanded blastocyst stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: trophectoderm |
Present |
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1E Merge Blastocyst
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1E SK3 Blastocyst
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Expression was predominately located at the trophectoderm cell membrane.
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Analysis Tools