Kcnn3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:320752 Lu YC, et al., Small-conductance calcium-activated K(+) channels 3 (SK3) regulate blastocyst hatching by control of intracellular calcium concentration. Hum Reprod. 2012 May;27(5):1421-30
Assay type:	RT-PCR
MGI Accession ID:	MGI:7495870
Gene symbol:	Kcnn3
Gene name:	potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3

Probe:	SK3-forward, SK3-reverse
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to 2-cell data.

Results

Image: 1C

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	101 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
Zygotes	E0.5 (a)	TS1: 1-cell stage conceptus	Absent	Not Specified	ICR		Not Specified
2-cell	E1.0 (a)	TS2: 2-cell stage conceptus	Present	Not Specified	ICR		Not Specified
4-cell	E2.0 (a)	TS3: 4-cell stage conceptus	Present	Not Specified	ICR		Not Specified
8-cell	E2.5 (a)	TS3: 8-cell stage conceptus	Present	Not Specified	ICR		Not Specified
Morula	E3.0 (a)	TS3: morula-stage conceptus	Present	Not Specified	ICR		Not Specified
Blastocyst	E3.5 (a)	TS4: blastocyst-stage conceptus	Present	Not Specified	ICR		Not Specified

Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) From 2-cell stage, expression level gradually increased during preimplantation embryo development, with the highest level observed in blastocysts.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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