Gene Expression Data
Assay Details
Assay
|
Reference:
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J:146976
Hao J, et al., Temporal and spatial localization of the dentin matrix proteins during dentin biomineralization. J Histochem Cytochem. 2009 Mar;57(3):227-37
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7494102
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Gene symbol:
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Dspp
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Gene name:
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dentin sialophosphoprotein
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Antibody:
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Anti-DSP
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
2E:
embryonic day 18.5
(more )
Specimen
2E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2E':
embryonic day 18.5
(more )
Specimen
2E':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3E:
postnatal day 1
(more )
Specimen
3E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: incisor |
odontoblast |
Present |
Regionally restricted |
3E'
,
3E
|
Expression was localized to secretory odontoblasts near the cuspal region.
|
Specimen
4E:
postnatal day 3
(more )
Specimen
4E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: incisor |
|
Present |
Regionally restricted |
4E
,
4E'
|
Expression was detected at the mineralization front. Most expression was found at the junction between the odontoblast cell body and the process.
|
TS27: incisor |
odontoblast |
Strong |
|
4E'
,
4E
|
High expression was in the cytoplasm.
|
TS27: incisor |
ameloblast |
Present |
|
4E
,
4E'
|
|
Specimen
5E:
postnatal day 3
(more )
Specimen
5E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: molar |
|
Present |
Regionally restricted |
5E'
,
5E
|
Expression was localized at the mineral front. Expression was mostly at the junction between the odontoblast cell body and the process.
|
TS27: molar |
odontoblast |
Present |
|
5E
,
5E'
|
|
TS27: alveolar process |
osteoblast |
Present |
|
5E''
,
5E
|
|
Specimen
6E:
postnatal day 5
(more )
Specimen
6E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: molar |
|
Strong |
Regionally restricted |
6E
,
6E'
|
Expression was localized at the mineral front.
|
TS28: molar |
odontoblast |
Strong |
|
6E
,
6E inset
|
Expression was localized throughout the cytoplasm.
|
TS28: molar dental pulp |
|
Present |
|
6E
,
6E'
|
Expression was localized in the nucleus of pulp cells.
|
TS28: dentin |
|
Present |
Regionally restricted |
6E
,
6E'
|
Expression was observed in the dentinal tubules.
|
Specimen
7E:
postnatal day 7
(more )
Specimen
7E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
8D:
postnatal day 20
(more )
Specimen
8D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 20
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: dentin |
|
Present |
Regionally restricted |
8D''
,
8D
,
8D'
|
Expression was observed in the dentinal tubules.
|
TS28: incisor |
odontoblast |
Present |
|
8D''
,
8D'
,
8D
|
Expression was detected around the plasma membrane.
|
Specimen
8E:
postnatal day 20
(more )
Specimen
8E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 20
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Table 1 E18:
embryonic day 18.0
(more )
Specimen
Table 1 E18:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Table 1 P1:
postnatal day 1
(more )
Specimen
Table 1 P1:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Table 1 P3:
postnatal day 3
(more )
Specimen
Table 1 P3:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Table 1 P5:
postnatal day 5
(more )
Specimen
Table 1 P5:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Table 1 P7:
postnatal day 7
(more )
Specimen
Table 1 P7:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Table 1 P20:
postnatal day 20
(more )
Specimen
Table 1 P20:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 20
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|