Cyp27b1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:203695 Lieben L, et al., Maternal hypervitaminosis D reduces fetal bone mass and mineral acquisition and leads to neonatal lethality. Bone. 2013 Nov;57(1):123-31
Assay type:	RT-PCR
MGI Accession ID:	MGI:7492486
Gene symbol:	Cyp27b1
Gene name:	cytochrome P450, family 27, subfamily b, polypeptide 1

Probe:	Cyp27b1-pF, Cyp27b1-pR
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Hprt1.

Results

Image: 1B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
+/+	E18.5	TS26: metanephros	Present	Not Specified; total RNA	involves: 129S1/Sv * 129X1/SvJ		Not Specified	Mother was Vdr+/+.
-/-	E18.5	TS26: metanephros	Present (a)	Not Specified; total RNA	involves: 129S1/Sv * 129X1/SvJ	Vdr^tm1.1Gcm/Vdr^tm1.1Gcm	Not Specified	Mother was Vdr-/-.
+/-	E18.5	TS26: metanephros	Trace (b)	Not Specified; total RNA	involves: 129S1/Sv * 129X1/SvJ	Vdr^tm1.1Gcm/Vdr⁺	Not Specified	Mother was Vdr-/-.

Notes:
(a) Expression was significantly reduced in this mutant compared to offspring from wild-type mothers.
(b) There was a 100-fold reduction in expression in this heterozygote compared to offspring from wild-type mothers.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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