Probe:	Chd2 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: embryo	Weak	Widespread	1C	Very low, widespread expression in many tissues.
TS20: brain	Weak	Diffuse	1C
TS20: spinal cord	Weak	Diffuse	1C
TS20: paraxial mesenchyme	Weak	Regionally restricted	1C	Diffuse expression in intersomitic regions.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Weak	Regionally restricted	1Ca	Little expression in neuroepithelium.

Structure	Level	Pattern	Image	Note
TS20: neocortex	Trace		1Cb	Little discernible expression.

Structure	Level	Pattern	Image	Note
TS22: embryo	Weak	Widespread	3C	Expression was low and widespread. Distinct expression patterns throughout embryo.
TS22: nervous system	Present		3C	Notably higher levels in developing nervous system.
TS22: incisor	Present		3C
TS22: olfactory epithelium	Present		3C
TS22: tongue	Present		3C
TS22: gut	Present		3C
TS22: hindlimb digit	Present		3C
TS22: liver	Present		3C
TS22: lung	Present		3C
TS22: metanephros	Present		3C
TS22: muscle tissue	Present		3C
TS22: thymus primordium	Present		3C
TS22: thyroid gland	Present		3C

Structure	Level	Pattern	Image	Note
TS22: cerebellum ventricular layer	Present		3Ca
TS22: rhombic lip	Present		3Ca

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong		3Cb

Structure	Level	Pattern	Image	Note
TS22: lens	Present		3Cc
TS22: neural retina	Present		3Cc
TS22: perioptic mesenchyme	Present		3Cc	Expression in anatomical space containing the optic nerve and its surrounding structures.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present		3Cd

Structure	Level	Pattern	Image	Note
TS22: cap mesenchyme	Present		3Ce	Expression in condensing mesenchyme of kidney.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present	Regionally restricted	4C	Expression highly localized in neocortex. Expression enriched in outer neocortex
TS27: hypothalamus	Present		4C
TS27: midbrain	Present		4C
TS27: pons	Present		4C
TS27: superior olivary nucleus	Present		4C
TS27: cerebellum	Present		4C

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present	Regionally restricted	4C'	Expression highly localized in neocortex. Expression enriched in outer neocortex
TS27: hypothalamus	Present		4C'
TS27: midbrain	Present		4C'
TS27: cerebellum	Present		4C'

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Moderate	Diffuse	4Ca

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present	Regionally restricted	4Cb	Expression enriched in outer neocortex.

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Widespread	5C
TS28: cerebellum	Present		5C
TS28: hippocampus	Present		5C
TS28: neocortex	Present		5C

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Widespread	5C'
TS28: cerebellum	Present		5C'
TS28: hippocampus	Present		5C'
TS28: main olfactory bulb	Present		5C'
TS28: neocortex	Present		5C'
TS28: rostral migratory stream	Present		5C'

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong		5Ca

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		5Cb
TS28: hippocampus CA1	Present		5Cb
TS28: hippocampus CA2	Present		5Cb
TS28: hippocampus CA3	Present		5Cb

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		5Cc
TS28: cortical layer III	Present		5Cc

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong		6C
TS28: hippocampus	Strong		6C
TS28: main olfactory bulb	Strong		6C
TS28: neocortex	Strong		6C
TS28: rostral migratory stream	Strong		6C

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong		6C'
TS28: hippocampus	Strong		6C'
TS28: neocortex	Strong		6C'

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		6Ca

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		6Cb
TS28: hippocampus CA1	Present		6Cb
TS28: hippocampus CA2	Present		6Cb
TS28: hippocampus CA3	Present		6Cb
TS28: hippocampus	Weak	Diffuse	6Cb	Expression in hippocampus lower and more diffuse compared to that of Chd8.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present		6Cc