Gene Expression Data
Assay Details
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Assay
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Reference:
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J:153218
Klinger S, et al., Loss of Erk3 function in mice leads to intrauterine growth restriction, pulmonary immaturity, and neonatal lethality. Proc Natl Acad Sci U S A. 2009 Sep 29;106(39):16710-5
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7489643
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Gene symbol:
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Mki67
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Gene name:
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antigen identified by monoclonal antibody Ki 67
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Antibody:
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anti-Ki67
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Quantification of cell proliferation was assessed by Mki67 immunostaining and are shown as a table in Figure 6.
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Results
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Specimen
E12.5 +/+:
embryonic day 12.5
(more  )
Specimen
E12.5 +/+:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lung |
Present |
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Specimen
E12.5 -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Mapk6tm1Melo/Mapk6tm1Melo
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lung |
Present |
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There is a decrease in the number of positive cells in this mutant compared to wild-type.
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Specimen
E14.5 +/+:
embryonic day 14.5
(more )
Specimen
E14.5 +/+:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung |
Present |
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Specimen
E14.5 -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Mapk6tm1Melo/Mapk6tm1Melo
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung |
Present |
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There is a decrease in the number of positive cells in this mutant compared to wild-type.
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Specimen
E16.5 +/+:
embryonic day 16.5
(more )
Specimen
E16.5 +/+:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lung |
Present |
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Specimen
E16.5 -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Mapk6tm1Melo/Mapk6tm1Melo
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lung |
Present |
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There is a significant decrease in the number of positive cells in this mutant compared to wild-type.
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Specimen
E18.5 +/+:
embryonic day 18.5
(more )
Specimen
E18.5 +/+:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung |
Present |
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Specimen
E18.5 -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Mapk6tm1Melo/Mapk6tm1Melo
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung |
Present |
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There is a 60% decrease in the number of positive cells in this mutant compared to wild-type.
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Analysis Tools