Gene Expression Data
Assay Details
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Assay
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Reference:
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J:127117
Pitteloud N, et al., Loss-of-function mutation in the prokineticin 2 gene causes Kallmann syndrome and normosmic idiopathic hypogonadotropic hypogonadism. Proc Natl Acad Sci U S A. 2007 Oct 30;104(44):17447-52
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7488190
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Gene symbol:
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Gnrh1
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Gene name:
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gonadotropin releasing hormone 1
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Results
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Specimen
5B:
embryonic day 13.5
(more  )
Note: Double labeled: red - Gnrh1; green - Prokr2 (assay MGI:7488193).
Specimen
5B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Gnrh1; green - Prokr2 (assay MGI:7488193).
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Specimen
5E:
embryonic day 13.5
(more )
Note: Double labeled: red - Gnrh1; green - Prokr2 (assay MGI:7488193).
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Gnrh1; green - Prokr2 (assay MGI:7488193).
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Specimen
5H:
postnatal adult
(more )
Note: Double labeled: red - Gnrh1; green - Prokr2 (assay MGI:7488193).
Specimen
5H:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Gnrh1; green - Prokr2 (assay MGI:7488193).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: preoptic area |
Present |
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5H
,
5I
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Gnrh1 expression did not colocalize with Prokr2.
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Specimen
6a:
embryonic day 13.5
(more )
Specimen
6a:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: nasal septum |
neuron |
Present |
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6a
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Some immunolabeled neurons were observed in the nasal septum.
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| TS21: main olfactory bulb |
neuron |
Present |
Regionally restricted |
6a
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Immunopositive migrating neurons passed through the olfactory bulb on the route to the preoptic area.
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| TS21: preoptic area |
neuron |
Present |
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6a
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Specimen
6b:
embryonic day 16.5
(more )
Specimen
6b:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: nasal septum |
neuron |
Present |
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6b
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Some immunolabeled neurons were observed in the nasal septum.
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| TS24: main olfactory bulb |
neuron |
Present |
Regionally restricted |
6b
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Immunopositive migrating neurons passed through the olfactory bulb on the route to the preoptic area.
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| TS24: preoptic area |
neuron |
Present |
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6b
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Specimen
6c:
embryonic day 18.5
(more )
Specimen
6c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6d:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Prok2tm1Qyz/Prok2tm1Qyz
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: nasal septum |
neuron |
Present |
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6d
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Many immunolabeled neurons were observed in the nasal septum of the mutant.
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| TS21: head |
neuron |
Present |
Regionally restricted |
6d
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After crossing the cribriform plate, immunopositive neurons were trapped in exogenous, spherical-shaped structures between the two olfactory bulbs in the mutant.
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Specimen
6e:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Prok2tm1Qyz/Prok2tm1Qyz
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: head |
neuron |
Present |
Regionally restricted |
6e
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After crossing the cribriform plate, immunopositive neurons were trapped in exogenous, spherical-shaped structures between the two olfactory bulbs in the mutant.
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Specimen
6f:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Prok2tm1Qyz/Prok2tm1Qyz
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: head |
neuron |
Present |
Regionally restricted |
6f
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After crossing the cribriform plate, immunopositive neurons were trapped in exogenous, spherical-shaped structures between the two olfactory bulbs in the mutant.
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Analysis Tools