Fgfr2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:167305 Sims-Lucas S, et al., Fgfr1 and the IIIc isoform of Fgfr2 play critical roles in the metanephric mesenchyme mediating early inductive events in kidney development. Dev Dyn. 2011 Jan;240(1):240-9
Assay type:	RNA in situ
MGI Accession ID:	MGI:7488107
Gene symbol:	Fgfr2
Gene name:	fibroblast growth factor receptor 2

Probe:	Fgfr2IIIb cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Controls were littermates.

Results

Specimen not shown Control wh mt: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: nephric duct	Strong

Specimen not shown Fgfr2IIIc-/- wh mt: embryonic day 10.5; Fgfr2^tm3.1Lni/Fgfr2^tm3.1Lni (more

)

Structure	Level	Pattern	Image	Note
TS17: nephric duct	Strong

Specimen not shown Fgfr1Mes-/-;Fgfr2IIIc-/- wh mt: embryonic day 10.5; Fgfr1^tm1Jpa/Fgfr1^tm1Jpa, Fgfr2^tm3.1Lni/Fgfr2^tm3.1Lni, Tg(Pax3-cre)1Joe/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: nephric duct	Strong

5A ©

Specimen 5A Control: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: nephric duct	Strong		5A
TS17: metanephric mesenchyme	Weak	Regionally restricted	5A	Expression is adjacent to the Wolffian duct.

5B ©

Specimen 5B: embryonic day 10.5; Fgfr1^tm1Jpa/Fgfr1^tm1Jpa, Fgfr2^tm3.1Lni/Fgfr2^tm3.1Lni, Tg(Pax3-cre)1Joe/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: nephric duct	Strong		5B
TS17: metanephric mesenchyme	Present	Regionally restricted	5B	Expression is adjacent to the Wolffian duct.

5C ©

Specimen 5C Control: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: cap mesenchyme	Present	Regionally restricted	5C	Expression is in cap mesenchyme surrounding the epithelial ureteric tips.

5D ©

Specimen 5D: embryonic day 12.5; Fgfr2^tm3.1Lni/Fgfr2^tm3.1Lni (more

)

Structure	Level	Pattern	Image	Note
TS20: cap mesenchyme	Present	Regionally restricted	5D	Expression in this mutant is the same as in controls.

Specimen not shown Control: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: primitive collecting duct	Strong

Specimen not shown Fgfr2IIIc-/-: embryonic day 12.5; Fgfr2^tm3.1Lni/Fgfr2^tm3.1Lni (more

)

Structure	Level	Pattern	Image	Note
TS20: primitive collecting duct	Strong

S2A ©

Specimen S2A Control: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: metanephros	Moderate	Regionally restricted	S2A	Expression is in stromal cells around the positive main ureteric trunk.
TS20: nephrogenic zone	Weak	Regionally restricted	S2A	Expression is in the cortical nephrogenic zone near the ureteric tips.

S2B ©

Specimen S2B Fgfr2IIIc-/-: embryonic day 12.5; Fgfr2^tm3.1Lni/Fgfr2^tm3.1Lni (more

)

Structure	Level	Pattern	Image	Note
TS20: metanephros	Strong	Regionally restricted	S2B	Expression is in stromal cells around the positive main ureteric trunk.
TS20: nephrogenic zone	Present	Regionally restricted	S2B	Expression is in the cortical nephrogenic zone near the ureteric tips.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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