About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:140337 Balastik M, et al., Deficiency in ubiquitin ligase TRIM2 causes accumulation of neurofilament light chain and neurodegeneration. Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):12016-21
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7487984
Gene symbol: Nefl
Gene name: neurofilament, light polypeptide
Antibody: NR4
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 5A: postnatal day 30 (more )
Structure Level Pattern Image Note
TS28: cerebellum white matter Present 5A

Specimen 5B: postnatal day 30; Trim2Gt(IRESBetageo)11Pgr/Trim2Gt(IRESBetageo)11Pgr (more )
Structure Level Pattern Image Note
TS28: cerebellum white matter Strong 5B Increased level of expression in cerebellar white matter compared to wild type.
TS28: cerebellum granule cell layer Present Regionally restricted 5B inset , 5B Expression in sporadic aggregates in granule cell layer.

Specimen 5C: postnatal day 62; Trim2Gt(IRESBetageo)11Pgr/Trim2Gt(IRESBetageo)11Pgr (more )
Note: Double labeled: red - Nefl; green - Calb1 (assay MGI:7487982).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell Present Regionally restricted 5C Expression in Purkinje cell axonal swellings co-localizing with Calb1 expression.

Specimen 5D: postnatal day 151 (more )
Structure Level Pattern Image Note
TS28: optic nerve Absent 5D

Specimen 5E: postnatal day 151; Trim2Gt(IRESBetageo)11Pgr/Trim2Gt(IRESBetageo)11Pgr (more )
Structure Level Pattern Image Note
TS28: optic nerve Present Regionally restricted 5E Expression in axonal swellings in optic nerve.

Specimen S8A: postnatal day 62 (more )
Structure Level Pattern Image Note
TS28: frontal cortex Present S8A

Specimen S8B: postnatal day 62; Trim2Gt(IRESBetageo)11Pgr/Trim2Gt(IRESBetageo)11Pgr (more )
Structure Level Pattern Image Note
TS28: frontal cortex Present Regionally restricted S8B Expression mostly in perikaryal inclusions in frontal cortex.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
05/07/2024
MGI 6.23
The Jackson Laboratory